Time-resolved Fluorescent Imaging of Glucose

Overview

Journal J Fluoresc

Specialties Biophysics
Chemistry

Date 2004 Dec 25

PMID 15617263

Citations 9

Authors

Michael Schaferling

Meng Wu

Otto S Wolfbeis

Affiliations

Soon will be listed here.

Abstract

A method for the fluorescent imaging of glucose is described that is based on the detection of enzymatically produced hydrogen peroxide, using the europium(III) tetracycline complex as the fluorescent probe incorporated into a hydrophilic polymer layer. Coadsorption of glucose oxidase (GOx) makes these sensor layers respond to the hydrogen peroxide produced by the GOx-assisted oxidation of glucose. The hydrogel layers are integrated into a 96-microwell plate for a parallel and simultaneous detection of various samples. Glucose is visualized by means of time resolved luminescence lifetime imaging. Unlike in previous methods, the determination of H2O2 does not require the addition of peroxidase or a catalyst to form a fluorescent product. The lifetime-based images obtained are compared with conventional fluorescence intensity-based methods with respect to sensitivity and the dynamic range of the sensor layer. The main advantages provided by this sensing scheme for H2O2 include reversibility, applicability at neutral pH, and the straightforwardness of the transducer system and the imaging device.

Citing Articles

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Fluorescence lifetime imaging of activatable target specific molecular probes.

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Optical properties of metacycline, oxytetracycline and chlortetracycline europium complexes in the presence of hydrogen peroxide.

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Fluorescence lifetime imaging system for in vivo studies.

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