Mnt1p and Mnt2p of Candida Albicans Are Partially Redundant Alpha-1,2-mannosyltransferases That Participate in O-linked Mannosylation and Are Required for Adhesion and Virulence

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2004 Nov 3

PMID 15519997

Citations 93

Authors

Carol A Munro

Steven Bates

Ed T Buurman

H Bleddyn Hughes

Donna M MacCallum

Gwyneth Bertram

Abdel Atrih

Michael A J Ferguson

Judith M Bain

Alexandra Brand

Suzanne Hamilton

Caroline Westwater

Lynn M Thomson

Alistair J P Brown

Frank C Odds

Neil A R Gow

Affiliations

Soon will be listed here.

Abstract

The MNT1 gene of the human fungal pathogen Candida albicans is involved in O-glycosylation of cell wall and secreted proteins and is important for adherence of C. albicans to host surfaces and for virulence. Here we describe the molecular analysis of CaMNT2, a second member of the MNT1-like gene family in C. albicans. Mnt2p also functions in O-glycosylation. Mnt1p and Mnt2p encode partially redundant alpha-1,2-mannosyltransferases that catalyze the addition of the second and third mannose residues in an O-linked mannose pentamer. Deletion of both copies of MNT1 and MNT2 resulted in reduction in the level of in vitro mannosyltransferase activity and truncation of O-mannan. Both the mnt2Delta and mnt1Delta single mutants were significantly reduced in adherence to human buccal epithelial cells and Matrigel-coated surfaces, indicating a role for O-glycosylated cell wall proteins or O-mannan itself in adhesion to host surfaces. The double mnt1Deltamnt2Delta mutant formed aggregates of cells that appeared to be the result of abnormal cell separation. The double mutant was attenuated in virulence, underlining the importance of O-glycosylation in pathogenesis of C. albicans infections.

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