Quantitative Detection of Methylated SOCS-1 , a Tumor Suppressor Gene, by a Modified Protocol of Quantitative Real Time Methylation-specific PCR Using SYBR Green and Its Use in Early Gastric Cancer Detection

Overview

Journal Biotechnol Lett

Specialties Biochemistry
Biotechnology

Date 2004 Oct 16

PMID 15483389

Citations 23

Authors

Michael W Y Chan

Eagle S H Chu

Ka-Fai To

Wai K Leung

Affiliations

Soon will be listed here.

Abstract

Although methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72 degrees C extension step. This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients.

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