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Localization of Antigen in Tissue Cells; Improvements in a Method for the Detection of Antigen by Means of Fluorescent Antibody

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Journal J Exp Med
Date 1950 Jan 1
PMID 15395569
Citations 491
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Abstract

Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.

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