Microscale Frictional Response of Bovine Articular Cartilage from Atomic Force Microscopy

Overview

Journal J Biomech

Specialty Physiology

Date 2004 Sep 25

PMID 15388310

Citations 39

Authors

Seonghun Park

Kevin D Costa

Gerard A Ateshian

Affiliations

Soon will be listed here.

Abstract

The objective of this study was to compare micro- and macroscale friction coefficients of bovine articular cartilage. Microscale measurements were performed using standard atomic force microscopy (AFM) techniques, using a 5 microm spherical probe tip. Twenty-four cylindrical osteochondral plugs were harvested in pairs from adjacent positions in six fresh bovine humeral heads (4-6 months old), and divided into two groups for AFM and macroscopic friction measurements. AFM measurements of friction were observed to be time-independent, whereas macroscale measurements demonstrated the well-documented time-dependent increase from a minimum to an equilibrium value. The microscale AFM friction coefficient (mu(AFM), 0.152+/-0.079) and macroscale equilibrium friction coefficient (mu(eq), 0.138+/-0.036) exhibited no statistical differences (p=0.50), while the macroscale minimum friction coefficient (mu(min), 0.004+/-0.001) was significantly smaller than mu(eq) and mu(AFM) (p<0.0001). Variations in articular surface roughness (Rq= 462+/-216 nm) did not correlate significantly with mu(AFM), mu(eq) or mu(min). The effective compressive modulus determined from AFM indentation tests using a Hertz contact analysis was E*=45.8+/-18.8 kPa. The main finding of this study is that mu(AFM) is more representative of the macroscale equilibrium friction coefficient, which represents the frictional response in the absence of cartilage interstitial fluid pressurization. These results suggest that AFM measurements may be highly suited for exploring the role of boundary lubricants in diarthrodial joint lubrication independently of the confounding effect of fluid pressurization to provide greater insight into articular cartilage lubrication.

Citing Articles

How Do Cartilage Lubrication Mechanisms Fail in Osteoarthritis? A Comprehensive Review.

Rajankunte Mahadeshwara M, Al-Jawad M, Hall R, Pandit H, El-Gendy R, Bryant M Bioengineering (Basel). 2024; 11(6).

PMID: 38927777 PMC: 11200606. DOI: 10.3390/bioengineering11060541.

Brushing Up on Cartilage Lubrication: Polyelectrolyte-Enhanced Tribological Rehydration.

Elkington R, Hall R, Beadling A, Pandit H, Bryant M Langmuir. 2024; 40(20):10648-10662.

PMID: 38712915 PMC: 11112737. DOI: 10.1021/acs.langmuir.4c00598.

Atomic Force Microscopy Micro-Indentation Methods for Determining the Elastic Modulus of Murine Articular Cartilage.

Arnold K, Sicard D, Tschumperlin D, Westendorf J Sensors (Basel). 2023; 23(4).

PMID: 36850434 PMC: 9967621. DOI: 10.3390/s23041835.

Attachment of cartilage wear particles to the synovium negatively impacts friction properties.

Estell E, Murphy L, Gangi L, Shah R, Ateshian G, Hung C J Biomech. 2021; 127:110668.

PMID: 34399243 PMC: 8492547. DOI: 10.1016/j.jbiomech.2021.110668.

AFM-Based Method for Measurement of Normal and Osteoarthritic Human Articular Cartilage Surface Roughness.

Ihnatouski M, Pauk J, Karev D, Karev B Materials (Basel). 2020; 13(10).

PMID: 32429426 PMC: 7288191. DOI: 10.3390/ma13102302.

References

Hills B . Boundary lubrication in vivo. Proc Inst Mech Eng H. 2000; 214(1):83-94. DOI: 10.1243/0954411001535264. View

Soltz M, Ateshian G . Interstitial fluid pressurization during confined compression cyclical loading of articular cartilage. Ann Biomed Eng. 2000; 28(2):150-9. DOI: 10.1114/1.239. View

Soltz M, Ateshian G . A Conewise Linear Elasticity mixture model for the analysis of tension-compression nonlinearity in articular cartilage. J Biomech Eng. 2001; 122(6):576-86. PMC: 2854000. DOI: 10.1115/1.1324669. View

Jay G, Tantravahi U, Britt D, Barrach H, Cha C . Homology of lubricin and superficial zone protein (SZP): products of megakaryocyte stimulating factor (MSF) gene expression by human synovial fibroblasts and articular chondrocytes localized to chromosome 1q25. J Orthop Res. 2001; 19(4):677-87. DOI: 10.1016/S0736-0266(00)00040-1. View

Kumar P, Oka M, Toguchida J, Kobayashi M, Uchida E, Nakamura T . Role of uppermost superficial surface layer of articular cartilage in the lubrication mechanism of joints. J Anat. 2001; 199(Pt 3):241-50. PMC: 1468328. DOI: 10.1046/j.1469-7580.2001.19930241.x. View

McMullen R, Kelty S . Investigation of human hair fibers using lateral force microscopy. Scanning. 2001; 23(5):337-45. DOI: 10.1002/sca.4950230507. View

Kim S, Marmo C, Somorjai G . Friction studies of hydrogel contact lenses using AFM: non-crosslinked polymers of low friction at the surface. Biomaterials. 2001; 22(24):3285-94. DOI: 10.1016/s0142-9612(01)00175-2. View

Mathur A, Collinsworth A, Reichert W, Kraus W, Truskey G . Endothelial, cardiac muscle and skeletal muscle exhibit different viscous and elastic properties as determined by atomic force microscopy. J Biomech. 2001; 34(12):1545-53. DOI: 10.1016/s0021-9290(01)00149-x. View

Habelitz S, Marshall S, Marshall Jr G, Balooch M . The functional width of the dentino-enamel junction determined by AFM-based nanoscratching. J Struct Biol. 2001; 135(3):294-301. DOI: 10.1006/jsbi.2001.4409. View

10.

Hu K, Radhakrishnan P, Patel R, Mao J . Regional structural and viscoelastic properties of fibrocartilage upon dynamic nanoindentation of the articular condyle. J Struct Biol. 2002; 136(1):46-52. DOI: 10.1006/jsbi.2001.4417. View

11.

GRANT L, Tiberg F . Normal and lateral forces between lipid covered solids in solution: correlation with layer packing and structure. Biophys J. 2002; 82(3):1373-85. PMC: 1301939. DOI: 10.1016/S0006-3495(02)75492-1. View

12.

Kim S, Opdahl A, Marmo C, Somorjai G . AFM and SFG studies of pHEMA-based hydrogel contact lens surfaces in saline solution: adhesion, friction, and the presence of non-crosslinked polymer chains at the surface. Biomaterials. 2002; 23(7):1657-66. DOI: 10.1016/s0142-9612(01)00292-7. View

13.

Dimitriadis E, Horkay F, Maresca J, Kachar B, Chadwick R . Determination of elastic moduli of thin layers of soft material using the atomic force microscope. Biophys J. 2002; 82(5):2798-810. PMC: 1302067. DOI: 10.1016/S0006-3495(02)75620-8. View

14.

Radmacher M . Measuring the elastic properties of living cells by the atomic force microscope. Methods Cell Biol. 2002; 68:67-90. DOI: 10.1016/s0091-679x(02)68005-7. View

15.

Wang C, Deng J, Ateshian G, Hung C . An automated approach for direct measurement of two-dimensional strain distributions within articular cartilage under unconfined compression. J Biomech Eng. 2002; 124(5):557-67. DOI: 10.1115/1.1503795. View

16.

Patel R, Mao J . Microstructural and elastic properties of the extracellular matrices of the superficial zone of neonatal articular cartilage by atomic force microscopy. Front Biosci. 2002; 8:a18-25. DOI: 10.2741/932. View

17.

Park S, Krishnan R, Nicoll S, Ateshian G . Cartilage interstitial fluid load support in unconfined compression. J Biomech. 2003; 36(12):1785-96. PMC: 2833094. DOI: 10.1016/s0021-9290(03)00231-8. View

18.

Mow V, Kuei S, Lai W, Armstrong C . Biphasic creep and stress relaxation of articular cartilage in compression? Theory and experiments. J Biomech Eng. 1980; 102(1):73-84. DOI: 10.1115/1.3138202. View

19.

Armstrong C, Lai W, Mow V . An analysis of the unconfined compression of articular cartilage. J Biomech Eng. 1984; 106(2):165-73. DOI: 10.1115/1.3138475. View

20.

Swann D, Silver F, Slayter H, Stafford W, Shore E . The molecular structure and lubricating activity of lubricin isolated from bovine and human synovial fluids. Biochem J. 1985; 225(1):195-201. PMC: 1144569. DOI: 10.1042/bj2250195. View