Persistence of Burkholderia Multivorans Within the Pulmonary Macrophage in the Murine Lung

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2004 Sep 24

PMID 15385520

Citations 6

Authors

Karen K Chu

Kelly L MacDonald

Donald J Davidson

David P Speert

Affiliations

Soon will be listed here.

Abstract

Differences in infection kinetics and host response between Burkholderia multivorans and Burkholderia cenocepacia were demonstrated in a pulmonary infection model in BALB/c mice. B. multivorans persisted in the lung, while B. cenocepacia was cleared. Indirect immunofluorescence and electron microscopy of B. multivorans-infected lungs localized bacteria to macrophages. Clearance of B. cenocepacia was associated with greater interleukin-1beta and neutrophil responses than the responses induced by B. multivorans.

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References

Sajjan U, Sylvester F, Forstner J . Cable-piliated Burkholderia cepacia binds to cytokeratin 13 of epithelial cells. Infect Immun. 2000; 68(4):1787-95. PMC: 97349. DOI: 10.1128/IAI.68.4.1787-1795.2000. View

Lugton I . Mucosa-associated lymphoid tissues as sites for uptake, carriage and excretion of tubercle bacilli and other pathogenic mycobacteria. Immunol Cell Biol. 1999; 77(4):364-72. DOI: 10.1046/j.1440-1711.1999.00836.x. View

Govan J . Infection control in cystic fibrosis: methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa and the Burkholderia cepacia complex. J R Soc Med. 2000; 93 Suppl 38:40-5. PMC: 1305883. View

Stotland P, Radzioch D, Stevenson M . Mouse models of chronic lung infection with Pseudomonas aeruginosa: models for the study of cystic fibrosis. Pediatr Pulmonol. 2000; 30(5):413-24. DOI: 10.1002/1099-0496(200011)30:5<413::aid-ppul8>3.0.co;2-9. View

Tomich M, Herfst C, Golden J, Mohr C . Role of flagella in host cell invasion by Burkholderia cepacia. Infect Immun. 2002; 70(4):1799-806. PMC: 127839. DOI: 10.1128/IAI.70.4.1799-1806.2002. View

Speert D, Henry D, Vandamme P, Corey M, Mahenthiralingam E . Epidemiology of Burkholderia cepacia complex in patients with cystic fibrosis, Canada. Emerg Infect Dis. 2002; 8(2):181-7. PMC: 3369581. DOI: 10.3201/eid0802.010163. View

Chu K, Davidson D, Halsey T, Chung J, Speert D . Differential persistence among genomovars of the Burkholderia cepacia complex in a murine model of pulmonary infection. Infect Immun. 2002; 70(5):2715-20. PMC: 127911. DOI: 10.1128/IAI.70.5.2715-2720.2002. View

Tomich M, Griffith A, Herfst C, Burns J, Mohr C . Attenuated virulence of a Burkholderia cepacia type III secretion mutant in a murine model of infection. Infect Immun. 2003; 71(3):1405-15. PMC: 148827. DOI: 10.1128/IAI.71.3.1405-1415.2003. View

Racz P, Tenner-Racz K, Myrvik Q, Shannon B, LOVE S . Sinus reactions in the hilar lymph node complex of rabbits undergoing a pulmonary cell-mediated immune response: sinus macrophage clumping reaction, sinus lymphocytosis and immature sinus histiocytosis. J Reticuloendothel Soc. 1978; 24(5):499-525. View

10.

Smith R, Bowman B, Speziale S . Interleukin-1 stimulates granule exocytosis from human neutrophils. Int J Immunopharmacol. 1986; 8(1):33-40. DOI: 10.1016/0192-0561(86)90070-6. View

11.

Breel M, van der Ende M, Sminia T, Kraal G . Subpopulations of lymphoid and non-lymphoid cells in bronchus-associated lymphoid tissue (BALT) of the mouse. Immunology. 1988; 63(4):657-62. PMC: 1454796. View

12.

Faccioli L, Souza G, Cunha F, Poole S, Ferreira S . Recombinant interleukin-1 and tumor necrosis factor induce neutrophil migration "in vivo" by indirect mechanisms. Agents Actions. 1990; 30(3-4):344-9. DOI: 10.1007/BF01966298. View

13.

Kolopp-Sarda M, Bene M, Massin N, Moulin J, Faure G . Immunohistological analysis of macrophages, B-cells, and T-cells in the mouse lung. Anat Rec. 1994; 239(2):150-7. DOI: 10.1002/ar.1092390205. View

14.

Walker D, Behzad A, Chu F . Neutrophil migration through preexisting holes in the basal laminae of alveolar capillaries and epithelium during streptococcal pneumonia. Microvasc Res. 1995; 50(3):397-416. DOI: 10.1006/mvre.1995.1067. View

15.

Brandolini L, Bertini R, Bizzarri C, Sergi R, Caselli G, Zhou D . IL-1 beta primes IL-8-activated human neutrophils for elastase release, phospholipase D activity, and calcium flux. J Leukoc Biol. 1996; 59(3):427-34. DOI: 10.1002/jlb.59.3.427. View

16.

Govan J, Deretic V . Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol Rev. 1996; 60(3):539-74. PMC: 239456. DOI: 10.1128/mr.60.3.539-574.1996. View

17.

Broxmeyer H, Cooper S, Cacalano G, Hague N, Bailish E, Moore M . Involvement of Interleukin (IL) 8 receptor in negative regulation of myeloid progenitor cells in vivo: evidence from mice lacking the murine IL-8 receptor homologue. J Exp Med. 1996; 184(5):1825-32. PMC: 2192871. DOI: 10.1084/jem.184.5.1825. View

18.

Hutchison M, Poxton I, Govan J . Burkholderia cepacia produces a hemolysin that is capable of inducing apoptosis and degranulation of mammalian phagocytes. Infect Immun. 1998; 66(5):2033-9. PMC: 108160. DOI: 10.1128/IAI.66.5.2033-2039.1998. View

19.

Sokol P, Darling P, Woods D, Mahenthiralingam E, Kooi C . Role of ornibactin biosynthesis in the virulence of Burkholderia cepacia: characterization of pvdA, the gene encoding L-ornithine N(5)-oxygenase. Infect Immun. 1999; 67(9):4443-55. PMC: 96763. DOI: 10.1128/IAI.67.9.4443-4455.1999. View

20.

Cheung D, Halsey K, Speert D . Role of pulmonary alveolar macrophages in defense of the lung against Pseudomonas aeruginosa. Infect Immun. 2000; 68(8):4585-92. PMC: 98382. DOI: 10.1128/IAI.68.8.4585-4592.2000. View