Functional Regulation of Osteoblastic Cells by the Interaction of Activin-A with Follistatin

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 1992 Mar 5

PMID 1537876

Citations 20

Authors

M Hashimoto

A Shoda

S Inoue

R Yamada

T Kondo

T Sakurai

N Ueno

M Muramatsu

Affiliations

Soon will be listed here.

Abstract

A high number of 125I-activin-A binding sites (an apparent Kd of 260 pM and 5,600 sites/cell) were observed on MC3T3-E1 cells, a well characterized osteoblastic cell line. Activin-A has a mitogenic effect on these cells, with the greatest influence being observed on cells in an undifferentiated state, as well as a suppressive effect on the alkaline phosphatase activity. Northern and ligand blotting analyses revealed that these osteoblastic cells produce follistatin, which was down-regulated by retinoic acid treatment. Because follistatin is an activin-A-binding protein, we suggest that activin-A modulates the function of osteoblastic cells by being regulated by follistatin during differentiation.

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