Circular Box C/D RNAs in Pyrococcus Furiosus

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2004 Sep 18

PMID 15375211

Citations 21

Authors

Natalia G Starostina

Sarah Marshburn

L Steven Johnson

Sean R Eddy

Rebecca M Terns

Michael P Terns

Affiliations

Soon will be listed here.

Abstract

Box C/D RNAs are small, noncoding RNAs that function in RNA modification in eukaryotes and archaea. Here, we report that box C/D RNAs exist in the rare biological form of RNA circles in the hyperthermophilic archaeon Pyrococcus furiosus. Northern analysis of box C/D RNAs reveals two prominent RNA species of different electrophoretic mobilities in total P. furiosus RNA preparations. Together, the results of Northern, ribozyme, RT-PCR, and lariat debranching analyses indicate that the two species are circular and linear RNAs of similar length and abundance. It seems that most, if not all, species of box C/D RNAs exist as circles in P. furiosus. In addition, the circular RNAs are found in complexes with proteins required for box C/D RNA function. Our finding places box C/D RNAs among the extremely few circular RNAs known to exist in nature. Moreover, the unexpected discovery of circular box C/D RNAs points to the existence of a previously unrecognized biogenesis pathway for box C/D RNAs in archaea.

Citing Articles

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Biogenesis and Regulatory Roles of Circular RNAs.

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Genetic and Functional Analyses of Archaeal ATP-Dependent RNA Ligase in C/D Box sRNA Circularization and Ribosomal RNA Processing.

Liu Y, Takagi Y, Sugijanto M, Nguyen K, Hirata A, Hori H Front Mol Biosci. 2022; 9:811548.

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