Fibrous Wound Repair Associated with Biodegradable Poly-L/D-lactide Copolymer Implants: Study of the Expression of Tenascin and Cellular Fibronectin

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2004 Sep 7

PMID 15348694

Citations 1

Authors

R Kontio

A Salo

R Suuronen

C Lindqvist

J H Meurman

I Virtanen

Affiliations

Soon will be listed here.

Abstract

Extracellular matrix (ECM) proteins are known to play a role in inflammatory and hyperplastic processes. Our aim in the present study was to study the distribution of tenascin (Tn), cellular fibronectins (cFn) and myofibroblasts around biodegradable poly-L/D-lactide (PLA) implants with monoclonal antibodies (MAb). Ethylene-oxide and gamma-irradiation sterilized PLA plate-type implants were inserted into the dorsal subcutaneous tissue of ten adult rabbits. Follow-up times were 4, 12, 16, 36 and 48 wk. Only some inflammatory cells were observed. In electron microscopy, a close coherence between the implant and the stromal tissue was seen. Immunoreactivity for Tn, cFn and alpha-actin was detected as a distinct layer bordering the implant, regardless of the sterilization method for the first 36 wk. From week 36 onwards, Tn immunoreactivity was downregulated while cFn immunoreactivity still persisted. A moderate upregulation for myofibroblasts was seen on the week 48 specimens, when hydrolysation of PLA implant had started. The persistent content of myofibroblasts, Tn and cFn suggests a prolonged wound healing produced by PLA implants. The absence of Tn at the week 48 specimens suggests that cFn, rather than Tn may be needed for alpha-actin-mediated contraction by myofibroblasts.

Citing Articles

The response of macrophages to particles of resorbable polymers and their degradation products.

Dawes E, Clarke S, Lamanuzzi N, Pinto E, Brooks R, Rushton N J Mater Sci Mater Med. 2004; 14(3):271-5.

PMID: 15348474 DOI: 10.1023/a:1022892925877.

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