Immunocytochemical Demonstration of Rod-opsin, S-antigen, and Neuron-specific Proteins in the Human Pineal Gland

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1992 Mar 1

PMID 1533347

Citations 14

Authors

S K Huang

D C Klein

H W Korf

Affiliations

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Abstract

The aim of this study was to examine whether rod-opsin and S-antigen immunoreactions were present in the pineal organ of adult man and how these immunoreactions were correlated with neuronal markers, e.g., synaptophysin, and neurofilaments L, H and M. Three perfusion-fixed epithalamic regions including the pineal organ and five pineal glands obtained at routine autopsy were used. The specimens were taken from female or male patients, 25 to 85 years of age. All immunoreactions were performed using highly specific, well-characterized antibodies. Rod-opsin and S-antigen-immunoreactive pinealocytes occurred in all pineal organs investigated; however, the immunoreaction was restricted to small subpopulations of pinealocytes (rod-opsin immunoreaction: approximately 3%-5%; S-antigen immunoreaction: approximately 5%-10% of the total population). In contrast, immunoreactions for synaptophysin and neurofilaments M and H were present in numerous pinealocytes. Immunoreactivity for neurofilament L was not found. These data suggest that the cellular composition of the human pineal organ is heterogeneous. Moreover, the presence of rod-opsin and S-antigen immunoreactions in the human pineal organ indicates that it may be affected by autoimmune retinal diseases that are provoked by antibodies against these proteins, as is the case in rodents and non-human primates.

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References

Korf H, Klein D, Zigler J, Gery I, Schachenmayr W . S-antigen-like immunoreactivity in a human pineocytoma. Acta Neuropathol. 1986; 69(1-2):165-7. DOI: 10.1007/BF00687054. View

Pfister C, Chabre M, Plouet J, Tuyen V, De Kozak Y, Faure J . Retinal S antigen identified as the 48K protein regulating light-dependent phosphodiesterase in rods. Science. 1985; 228(4701):891-3. DOI: 10.1126/science.2988124. View

Collins V . Pineocytoma with neuronal differentiation demonstrated immunocytochemically. A case report. Acta Pathol Microbiol Immunol Scand A. 1987; 95(3):113-7. DOI: 10.1111/j.1699-0463.1987.tb00016_95a.x. View

Foster R, Timmers A, Schalken J, de Grip W . A comparison of some photoreceptor characteristics in the pineal and retina. II. The Djungarian hamster (Phodopus sungorus). J Comp Physiol A. 1989; 165(4):565-72. DOI: 10.1007/BF00611242. View

Korf H, Sato T, Oksche A . Complex relationships between the pineal organ and the medial habenular nucleus-pretectal region of the mouse as revealed by S-antigen immunocytochemistry. Cell Tissue Res. 1990; 261(3):493-500. DOI: 10.1007/BF00313528. View

Muller B, Peichl L, de Grip W, Gery I, Korf H . Opsin- and S-antigen-like immunoreactions in photoreceptors of the tree shrew retina. Invest Ophthalmol Vis Sci. 1989; 30(3):530-5. View

Korf H, Moller M, Gery I, Zigler J, Klein D . Immunocytochemical demonstration of retinal S-antigen in the pineal organ of four mammalian species. Cell Tissue Res. 1985; 239(1):81-5. DOI: 10.1007/BF00214906. View

Hirose S, Singh V, Donoso L, Shinohara T, Kotake S, Tanaka T . An 18-mer peptide derived from the retinal S antigen induces uveitis and pinealitis in primates. Clin Exp Immunol. 1989; 77(1):106-11. PMC: 1541935. View

Kalsow C, WACKER W . Localization of a uveitogenic soluble retinal antigen in the normal guinea pig eye by an indirect fluorescent antibody technique. Int Arch Allergy Appl Immunol. 1973; 44(1):11-20. DOI: 10.1159/000230914. View

10.

Korf H, Bruce J, Vistica B, Rollag M, STEIN B, Klein D . Immunoreactive S-antigen in cerebrospinal fluid: a marker of pineal parenchymal tumors?. J Neurosurg. 1989; 70(5):682-7. DOI: 10.3171/jns.1989.70.5.0682. View

11.

Tsin A, Phillips T, Reiter R . An evaluation on the level of retinoids in the bovine pineal body. Adv Pineal Res. 2015; 3:147-150. PMC: 4564015. View

12.

Rodrigues M, Hackett J, Gaskins R, Wiggert B, Lee L, Redmond M . Interphotoreceptor retinoid-binding protein in retinal rod cells and pineal gland. Invest Ophthalmol Vis Sci. 1986; 27(5):844-50. View

13.

van Veen T, Elofsson R, Hartwig H, Gery I, Mochizuki M, Cena V . Retinal S-antigen: immunocytochemical and immunochemical studies on distribution in animal photoreceptors and pineal organs. Exp Biol. 1986; 45(1):15-25. View

14.

Korf H, Foster R, Ekstrom P, Schalken J . Opsin-like immunoreaction in the retinae and pineal organs of four mammalian species. Cell Tissue Res. 1985; 242(3):645-8. DOI: 10.1007/BF00225432. View

15.

Redecker P, GRUBE D, Jahn R . Immunohistochemical localization of synaptophysin (p38) in the pineal gland of the Mongolian gerbil (Meriones unguiculatus). Anat Embryol (Berl). 1990; 181(5):433-40. DOI: 10.1007/BF02433790. View

16.

van Veen T, Katial A, Shinohara T, Barrett D, Wiggert B, Chader G . Retinal photoreceptor neurons and pinealocytes accumulate mRNA for interphotoreceptor retinoid-binding protein (IRBP). FEBS Lett. 1986; 208(1):133-7. DOI: 10.1016/0014-5793(86)81547-2. View

17.

Rodriguez E, Korf H, Oksche A, Yulis C, Hein S . Pinealocytes immunoreactive with antisera against secretory glycoproteins of the subcommissural organ: a comparative study. Cell Tissue Res. 1988; 254(3):469-80. DOI: 10.1007/BF00226496. View

18.

Perentes E, Rubinstein L, Herman M, Donoso L . S-antigen immunoreactivity in human pineal glands and pineal parenchymal tumors. A monoclonal antibody study. Acta Neuropathol. 1986; 71(3-4):224-7. DOI: 10.1007/BF00688043. View

19.

Dizhoor A, Ray S, Kumar S, Niemi G, Spencer M, Brolley D . Recoverin: a calcium sensitive activator of retinal rod guanylate cyclase. Science. 1991; 251(4996):915-8. DOI: 10.1126/science.1672047. View

20.

Palczewski K, Carruth M, Adamus G, McDowell J, HARGRAVE P . Molecular, enzymatic and functional properties of rhodopsin kinase from rat pineal gland. Vision Res. 1990; 30(8):1129-37. DOI: 10.1016/0042-6989(90)90170-p. View