Immunoglobulin Heavy-chain and CD3 Delta-chain Gene Enhancers Are DNase I-hypersensitive in Hemopoietic Progenitor Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1992 Apr 15

PMID 1533043

Citations 10

Authors

A M Ford

C A Bennett

L E Healy

E Navarro

E Spooncer

M F Greaves

Affiliations

Soon will be listed here.

Abstract

Multipotential interleukin 3-dependent non-immortalized murine hemopoietic progenitor cells have DNase I-hypersensitive sites in the immunoglobulin heavy-chain and CD3 delta enhancers and transcribe germ-line T-cell antigen receptor gamma-chain (TCR gamma), but not IgM or TCR beta, genes. Induction of myeloid differentiation in these cells clones down expression and/or transcriptional accessibility of the immunoglobulin heavy-chain and TCR gamma genes. The CD3 delta enhancer region remains DNase I-hypersensitive but closes down in B cells. In embryonic stem cells and pan-mesodermal cells, these genes or enhancer regions are neither expressed nor DNase I-hypersensitive. These data suggest that lineage potential may be programmed, at least in part, by alterations in the accessibility or conformation of regulatory regions of genes and that some promiscuity of gene expression and/or accessibility can precede lineage commitment and maturation in progenitor cells induced to self-renew by interleukin 3.

Citing Articles

Gene set control analysis predicts hematopoietic control mechanisms from genome-wide transcription factor binding data.

Joshi A, Hannah R, Diamanti E, Gottgens B Exp Hematol. 2012; 41(4):354-66.e14.

PMID: 23220237 PMC: 3630327. DOI: 10.1016/j.exphem.2012.11.008.

Global gene expression reveals a set of new genes involved in the modification of cells during erythroid differentiation.

da Cunha A, Brugnerotto A, Duarte A, Lanaro C, Costa G, Saad S Cell Prolif. 2010; 43(3):297-309.

PMID: 20546246 PMC: 6496675. DOI: 10.1111/j.1365-2184.2010.00679.x.

Spatial organization of gene expression: the active chromatin hub.

de Laat W, Grosveld F Chromosome Res. 2003; 11(5):447-59.

PMID: 12971721 DOI: 10.1023/a:1024922626726.

Marrow stem cells shift gene expression and engraftment phenotype with cell cycle transit.

Lambert J, Liu M, Colvin G, Dooner M, McAuliffe C, Becker P J Exp Med. 2003; 197(11):1563-72.

PMID: 12782718 PMC: 2193900. DOI: 10.1084/jem.20030031.

The new stem cell biology.

Quesenberry P, Colvin G, Lambert J, Frimberger A, Dooner M, McAuliffe C Trans Am Clin Climatol Assoc. 2002; 113:182-206; discussion 206-7.

PMID: 12053709 PMC: 2194475.

References

Burgess A, Camakaris J, Metcalf D . Purification and properties of colony-stimulating factor from mouse lung-conditioned medium. J Biol Chem. 1977; 252(6):1998-2003. View

Dexter T . Stromal cell associated haemopoiesis. J Cell Physiol Suppl. 1982; 1:87-94. DOI: 10.1002/jcp.1041130414. View

Caplan A, Ordahl C . Irreversible gene repression model for control of development. Science. 1978; 201(4351):120-30. DOI: 10.1126/science.351805. View

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View

Dexter T, ALLEN T, Scott D, Teich N . Isolation and characterisation of a bipotential haematopoietic cell line. Nature. 1979; 277(5696):471-4. DOI: 10.1038/277471a0. View

Heyworth C, Dexter T, Kan O, Whetton A . The role of hemopoietic growth factors in self-renewal and differentiation of IL-3-dependent multipotential stem cells. Growth Factors. 1990; 2(2-3):197-211. DOI: 10.3109/08977199009071506. View

Gordon M . Hemopoietic growth factors and receptors: bound and free. Cancer Cells. 1991; 3(4):127-33. View

Pevny L, Simon M, Robertson E, Klein W, Tsai S, DAgati V . Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1. Nature. 1991; 349(6306):257-60. DOI: 10.1038/349257a0. View

Su L, Kadesch T . The immunoglobulin heavy-chain enhancer functions as the promoter for I mu sterile transcription. Mol Cell Biol. 1990; 10(6):2619-24. PMC: 360620. DOI: 10.1128/mcb.10.6.2619-2624.1990. View

10.

Hoatlin M, Kozak S, Lilly F, Chakraborti A, Kozak C, Kabat D . Activation of erythropoietin receptors by Friend viral gp55 and by erythropoietin and down-modulation by the murine Fv-2r resistance gene. Proc Natl Acad Sci U S A. 1990; 87(24):9985-9. PMC: 55299. DOI: 10.1073/pnas.87.24.9985. View

11.

Wotton D, Flanagan B, Owen M . Chromatin configuration of the human CD2 gene locus during T-cell development. Proc Natl Acad Sci U S A. 1989; 86(11):4195-9. PMC: 287417. DOI: 10.1073/pnas.86.11.4195. View

12.

Blackman M, Koshland M . Specific 5' and 3' regions of the mu-chain gene are undermethylated at distinct stages of B-cell differentiation. Proc Natl Acad Sci U S A. 1985; 82(11):3809-13. PMC: 397877. DOI: 10.1073/pnas.82.11.3809. View

13.

Ford A, Watt S, Furley A, Molgaard H, Greaves M . Cell lineage specificity of chromatin configuration around the immunoglobulin heavy chain enhancer. EMBO J. 1988; 7(8):2393-9. PMC: 457105. DOI: 10.1002/j.1460-2075.1988.tb03084.x. View

14.

Georgopoulos K, van den Elsen P, Bier E, Maxam A, Terhorst C . A T cell-specific enhancer is located in a DNase I-hypersensitive area at the 3' end of the CD3-delta gene. EMBO J. 1988; 7(8):2401-7. PMC: 457106. DOI: 10.1002/j.1460-2075.1988.tb03085.x. View

15.

Alt F, Blackwell T, DePinho R, Reth M, Yancopoulos G . Regulation of genome rearrangement events during lymphocyte differentiation. Immunol Rev. 1986; 89:5-30. DOI: 10.1111/j.1600-065x.1986.tb01470.x. View

16.

Yancopoulos G, Alt F . Regulation of the assembly and expression of variable-region genes. Annu Rev Immunol. 1986; 4:339-68. DOI: 10.1146/annurev.iy.04.040186.002011. View

17.

Furley A, Mizutani S, Weilbaecher K, Dhaliwal H, Ford A, Chan L . Developmentally regulated rearrangement and expression of genes encoding the T cell receptor-T3 complex. Cell. 1986; 46(1):75-87. DOI: 10.1016/0092-8674(86)90861-5. View

18.

Holmes K, PIERCE J, Davidson W, Morse 3rd H . Murine hematopoietic cells with pre-B or pre-B/myeloid characteristics are generated by in vitro transformation with retroviruses containing fes, ras, abl, and src oncogenes. J Exp Med. 1986; 164(2):443-57. PMC: 2188240. DOI: 10.1084/jem.164.2.443. View

19.

Spooncer E, Heyworth C, Dunn A, Dexter T . Self-renewal and differentiation of interleukin-3-dependent multipotent stem cells are modulated by stromal cells and serum factors. Differentiation. 1986; 31(2):111-8. DOI: 10.1111/j.1432-0436.1986.tb00391.x. View

20.

Furley A, Reeves B, Mizutani S, Altass L, Watt S, Jacob M . Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines. Blood. 1986; 68(5):1101-7. View