Selectivity and Affinity of Triplex-forming Oligonucleotides Containing 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine for Recognizing AT Base Pairs in Duplex DNA

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2004 Aug 20

PMID 15317869

Citations 9

Authors

Sadie D Osborne

Vicki E C Powers

David A Rusling

Oliver Lack

Keith R Fox

Tom Brown

Affiliations

Soon will be listed here.

Abstract

We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circular dichroism to demonstrate that, in the parallel triplex binding motif, 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) has very high affinity for AT relative to all other Watson-Crick base pairs in DNA. Complexes containing two or more substitutions with this nucleotide analogue are stable at pH 7.0, even though they contain several C.GC base triplets. These modified triplex-forming oligonucleotides retain exquisite sequence specificity, with enhanced discrimination against YR base pairs (especially CG). These properties make BAU a useful base analogue for the sequence-specific creation of stable triple helices at pH 7.0.

Citing Articles

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