Phylogenetic Analysis of Methyl Coenzyme-M Reductase Detected from the Bovine Rumen

Overview

Journal Lett Appl Microbiol

Publisher Oxford University Press

Specialty Microbiology

Date 2004 Aug 4

PMID 15287871

Citations 11

Authors

N Tatsuoka

N Mohammed

M Mitsumori

K Hara

M Kurihara

H Itabashi

Affiliations

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Abstract

Aims: The object of the present study is isolation of methyl coenzyme-M reductase (MCR) genes (mcrA) from the bovine rumen fluid and determination of phylogenetical placements of the genes to investigate mechanisms of methanogenesis in the rumen from a point of view of mcrA genes.

Methods: Genes for methanogen-specific MCR were isolated from the bovine rumen by PCR amplification. The deduced amino acid sequences were fitted to the alignments of mcrA gene products from the referred sequences.

Significance And Impact Of The Study: Although the deduced amino acid sequences of mcrA genes, isolated from the bovine rumen in the present study, were close to that of Methanobrevibacter ruminantium, these amino acid sequences did not fall into known clusters of MCR. The findings suggest that methanogenesis in the rumen would be partially carried out by unknown methanogens.

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Comparative analysis of methanogen diversity in the rumen of crossbred buffalo and cattle in the Philippines by using the functional gene mcrA.

Lwin K, Matsui H, Ban-Tokuda T, Kondo M, Lapitan R, Herrera J Mol Biol Rep. 2012; 39(12):10769-74.

PMID: 23053974 DOI: 10.1007/s11033-012-1969-1.