Genome-wide Search for Loss of Heterozygosity in Chinese Patients with Sporadic Colorectal Cancer

Overview

Journal Int J Gastrointest Cancer

Date 2004 Jul 6

PMID 15235134

Citations 7

Authors

Zhihai Peng

Fang Zhang

Chongzhi Zhou

Yun Ling

Shaochun Bai

Wanqing Liu

Guoqiang Qiu

Lin He

Liwei Wang

Daoyan Wei

Edward Lin

Keping Xie

Affiliations

Soon will be listed here.

Abstract

In an attempt to integrally investigate the loss of tumor suppressor genes and search for putative suppressor loci associated with tumor occurrence and progression, we conducted a genome-wide loss of hetero zygosity (LOH) study of 83 tumor samples obtained from Chinese patients with sporadic colorectal cancer. We employed 400 fluorescence-labeled microsatellite marker primers to amplify the corresponding loci of the genomic DNA and then electrophoresed the polymerase chain reaction products and analyzed the fluorescent signals. The LOH frequencies were high (>35%) but were not associated with the tumor stage and progression in 20 loci, including the regions where TP53, E-cadherin, deleted in colorectal carcinoma (DCC), phosphatase and tensin homolog deleted on chromosome 10 (PTEN), mothers against decapentaplegic, Drosophila, homolog of 2 (MADH2) and mothers against decapentaplegic, Drosophila, homolog of 4 (MADH4) reside. Loss of other loci, including two narrow regions on chromosome 2, was found to relate to the tumor stage, suggesting that this genomic instability may contribute to tumor progression.

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References

Rothman R, Mahboubi A, REID A, Kim C, DeCosta B, JACOBSON A . [3H]1,3-Di(2-tolyl)guanidine labels two high affinity binding sites in guinea pig brain: evidence for allosteric regulation by calcium channel blockers and sigma ligands. NIDA Res Monogr. 1991; 105:335-6. View

Valarmathi M, A A, Deo S, Shukla N, Das S . BRCA1 germline mutations in Indian familial breast cancer. Hum Mutat. 2002; 21(1):98-9. DOI: 10.1002/humu.9099. View

Isobe M, Emanuel B, Givol D, Oren M, Croce C . Localization of gene for human p53 tumour antigen to band 17p13. Nature. 1986; 320(6057):84-5. DOI: 10.1038/320084a0. View

Berney C, Fisher R, Yang J, Russell P, Crowe P . Genomic alterations (LOH, MI) on chromosome 17q21-23 and prognosis of sporadic colorectal cancer. Int J Cancer. 2000; 89(1):1-7. DOI: 10.1002/(sici)1097-0215(20000120)89:1<1::aid-ijc1>3.0.co;2-7. View

Nakamura Y, Lathrop M, Leppert M, Dobbs M, Wasmuth J, Wolff E . Localization of the genetic defect in familial adenomatous polyposis within a small region of chromosome 5. Am J Hum Genet. 1988; 43(5):638-44. PMC: 1715544. View

Powell S, Zilz N, Bryan T, Hamilton S, Thibodeau S, Vogelstein B . APC mutations occur early during colorectal tumorigenesis. Nature. 1992; 359(6392):235-7. DOI: 10.1038/359235a0. View

Sato T, Tanigami A, Yamakawa K, Akiyama F, Kasumi F, Sakamoto G . Allelotype of breast cancer: cumulative allele losses promote tumor progression in primary breast cancer. Cancer Res. 1990; 50(22):7184-9. View

Tarafa G, Villanueva A, Farre L, Rodriguez J, Musulen E, Reyes G . DCC and SMAD4 alterations in human colorectal and pancreatic tumor dissemination. Oncogene. 2000; 19(4):546-55. DOI: 10.1038/sj.onc.1203353. View

Langston A, Malone K, Thompson J, Daling J, Ostrander E . BRCA1 mutations in a population-based sample of young women with breast cancer. N Engl J Med. 1996; 334(3):137-42. DOI: 10.1056/NEJM199601183340301. View

10.

Vasen H, Watson P, Mecklin J, Lynch H . New clinical criteria for hereditary nonpolyposis colorectal cancer (HNPCC, Lynch syndrome) proposed by the International Collaborative group on HNPCC. Gastroenterology. 1999; 116(6):1453-6. DOI: 10.1016/s0016-5085(99)70510-x. View

11.

Steck P, Pershouse M, Jasser S, Yung W, Lin H, Ligon A . Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat Genet. 1997; 15(4):356-62. DOI: 10.1038/ng0497-356. View

12.

van der Heijden O, Chiu H, Park T, Takahashi H, Livolsi V, Risinger J . Allelotype analysis of uterine leiomyoma: localization of a potential tumor suppressor gene to a 4-cM region of chromosome 7q. Mol Carcinog. 1998; 23(4):243-7. DOI: 10.1002/(sici)1098-2744(199812)23:4<243::aid-mc7>3.0.co;2-e. View

13.

Cho K, Oliner J, Simons J, Hedrick L, Fearon E, Preisinger A . The DCC gene: structural analysis and mutations in colorectal carcinomas. Genomics. 1994; 19(3):525-31. DOI: 10.1006/geno.1994.1102. View

14.

Hoshino Y, Horikawa I, Oshimura M, Yuasa Y . Normal human chromosome 5, on which a familial adenomatous polyposis gene is located, has tumor suppressive activity. Biochem Biophys Res Commun. 1991; 174(1):298-304. DOI: 10.1016/0006-291x(91)90520-h. View

15.

Fearon E . Molecular genetics of colorectal cancer. Ann N Y Acad Sci. 1995; 768:101-10. DOI: 10.1111/j.1749-6632.1995.tb12114.x. View

16.

Zhou C, Peng Z, Zhang F, Qiu G, He L . Loss of heterozygosity on long arm of chromosome 22 in sporadic colorectal carcinoma. World J Gastroenterol. 2002; 8(4):668-73. PMC: 4656318. DOI: 10.3748/wjg.v8.i4.668. View

17.

Rougier P, Mitry E . Epidemiology, treatment and chemoprevention in colorectal cancer. Ann Oncol. 2003; 14 Suppl 2:ii3-5. DOI: 10.1093/annonc/mdg722. View

18.

Sieben N, ter Haar N, Cornelisse C, Fleuren G, Cleton-Jansen A . PCR artifacts in LOH and MSI analysis of microdissected tumor cells. Hum Pathol. 2000; 31(11):1414-9. View

19.

Kang J, Essig Y, Mao W, Irby R, Wloch M, Yeatman T . Analysis of colorectal cancer by comparative genomic hybridization: evidence for induction of the metastatic phenotype by loss of tumor suppressor genes. Clin Cancer Res. 1998; 4(4):879-86. View

20.

Li J, Yen C, Liaw D, Podsypanina K, Bose S, Wang S . PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science. 1997; 275(5308):1943-7. DOI: 10.1126/science.275.5308.1943. View