MicroRNA-34a Functions As a Tumor Suppressor by Directly Targeting Oncogenic PLCE1 in Kazakh Esophageal Squamous Cell Carcinoma

Overview

Journal Oncotarget

Specialty Oncology

Date 2017 Dec 2

PMID 29190930

Citations 20

Authors

Xiao-Bin Cui

Hao Peng

Ran-Ran Li

Jian-Qin Mu

Lan Yang

Na Li

Chun-Xia Liu

Jian-Ming Hu

Shu-Gang Li

Yutao Wei

Laibo-Yin

Hong Zhou

Feng Li

Yun-Zhao Chen

Affiliations

Soon will be listed here.

Abstract

Esophageal squamous cell carcinoma (ESCC) is one of the frequent malignant tumors with poor prognosis worldwide. Identifying the prognostic biomarkers and potential mechanisms of such tumors has attracted increasing interest in esophageal cancer biology. Our previous study showed that phospholipase C elipson 1 (PLCE1) expression is up-regulated and associated with disease progression in esophageal carcinoma. MicroRNAs (miRNAs) play vital roles in regulating its target gene expression. However, studies on miRNA-regulated PLCE1 expression and its cellular function are still very few. We found that miR-34a is significantly expressed lower in ESCC tissues. We further showed that PLCE1 is a direct functional target gene of miR-34a, and the functional roles of miR-34a in ESCC cell lines were also determined through gain- and loss-of-function analyses. Results revealed that miR-34a functions as a tumor suppressor by inhibiting the proliferation, migration, and EMT phenotype, as well as promoting apoptosis of ESCC cell lines. Moreover, PLCE1 is overexpressed in ESCC tumors and promotes tumorigenicity and vitro. PLCE1 expression is negatively correlated with miR-34a profiles in ESCC tissues. Our data suggest that miR-34a exerts its anti-cancer function by suppressing PLCE1. The newly identified miR-34a/PLCE1 axis partially illustrates the molecular mechanism of ESCC metastasis and represents a new candidate therapeutic target for ESCC treatment.

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