Efficient Production of Germline Transgenic Chickens Using Lentiviral Vectors

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2004 Jun 12

PMID 15192698

Citations 137

Authors

Michael J McGrew

Adrian Sherman

Fiona M Ellard

Simon G Lillico

Hazel J Gilhooley

Alan J Kingsman

Kyriacos A Mitrophanous

Helen Sang

Affiliations

Soon will be listed here.

Abstract

An effective method for genetic modification of chickens has yet to be developed. An efficient technology, enabling production of transgenic birds at high frequency and with reliable expression of transgenes, will have many applications, both in basic research and in biotechnology. We investigated the efficiency with which lentiviral vectors could transduce the chicken germ line and examined the expression of introduced reporter transgenes. Ten founder cockerels transmitted the vector to between 4% and 45% of their offspring and stable transmission to the G2 generation was demonstrated. Analysis of expression of reporter gene constructs in several transgenic lines showed a conserved expression profile between individuals that was maintained after transmission through the germ line. These data demonstrate that lentiviral vectors can be used to generate transgenic lines with an efficiency in the order of 100-fold higher than any previously published method, with no detectable silencing of transgene expression between generations.

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References

Zhan Y, Brady J, Johnston A, Lew A . Predominant transgene expression in exocrine pancreas directed by the CMV promoter. DNA Cell Biol. 2000; 19(11):639-45. DOI: 10.1089/10445490050199045. View

Bienemann A, Martin-Rendon E, Cosgrave A, Glover C, Wong L, Kingsman S . Long-term replacement of a mutated nonfunctional CNS gene: reversal of hypothalamic diabetes insipidus using an EIAV-based lentiviral vector expressing arginine vasopressin. Mol Ther. 2003; 7(5 Pt 1):588-96. DOI: 10.1016/s1525-0016(03)00069-8. View

Bosselman R, Hsu R, BOGGS T, Hu S, BRUSZEWSKI J, Ou S . Germline transmission of exogenous genes in the chicken. Science. 1989; 243(4890):533-5. DOI: 10.1126/science.2536194. View

Corcoran J, So P, Barber R, Vincent K, Mazarakis N, Mitrophanous K . Retinoic acid receptor beta2 and neurite outgrowth in the adult mouse spinal cord in vitro. J Cell Sci. 2002; 115(Pt 19):3779-86. DOI: 10.1242/jcs.00046. View

Furth P, Hennighausen L, Baker C, Beatty B, Woychick R . The variability in activity of the universally expressed human cytomegalovirus immediate early gene 1 enhancer/promoter in transgenic mice. Nucleic Acids Res. 1991; 19(22):6205-8. PMC: 329125. DOI: 10.1093/nar/19.22.6205. View

Hofmann A, Kessler B, Ewerling S, Weppert M, Vogg B, Ludwig H . Efficient transgenesis in farm animals by lentiviral vectors. EMBO Rep. 2003; 4(11):1054-60. PMC: 1326377. DOI: 10.1038/sj.embor.embor7400007. View

Urven L, Erickson C, ABBOTT U, McCarrey J . Analysis of germ line development in the chick embryo using an anti-mouse EC cell antibody. Development. 1988; 103(2):299-304. DOI: 10.1242/dev.103.2.299. View

Rohll J, Mitrophanous K, Martin-Rendon E, Ellard F, Radcliffe P, Mazarakis N . Design, production, safety, evaluation, and clinical applications of nonprimate lentiviral vectors. Methods Enzymol. 2002; 346:466-500. DOI: 10.1016/s0076-6879(02)46072-7. View

EYAL-GILADI H, Kochav S . From cleavage to primitive streak formation: a complementary normal table and a new look at the first stages of the development of the chick. I. General morphology. Dev Biol. 1976; 49(2):321-37. DOI: 10.1016/0012-1606(76)90178-0. View

10.

Lois C, Hong E, Pease S, Brown E, Baltimore D . Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science. 2002; 295(5556):868-72. DOI: 10.1126/science.1067081. View

11.

Naldini L, Blomer U, Gallay P, Ory D, Mulligan R, Gage F . In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science. 1996; 272(5259):263-7. DOI: 10.1126/science.272.5259.263. View

12.

Pfeifer A, Ikawa M, Dayn Y, Verma I . Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci U S A. 2002; 99(4):2140-5. PMC: 122332. DOI: 10.1073/pnas.251682798. View

13.

Mizuarai S, Ono K, Yamaguchi K, Nishijima K, Kamihira M, Iijima S . Production of transgenic quails with high frequency of germ-line transmission using VSV-G pseudotyped retroviral vector. Biochem Biophys Res Commun. 2001; 286(3):456-63. DOI: 10.1006/bbrc.2001.5422. View

14.

Sherman A, Dawson A, Mather C, Gilhooley H, Li Y, Mitchell R . Transposition of the Drosophila element mariner into the chicken germ line. Nat Biotechnol. 1998; 16(11):1050-3. DOI: 10.1038/3497. View

15.

Salter D, Crittenden L . Artificial insertion of a dominant gene for resistance to avian leukosis virus into the germ line of the chicken. Theor Appl Genet. 2013; 77(4):457-61. DOI: 10.1007/BF00274263. View

16.

Sang H . Transgenic chickens--methods and potential applications. Trends Biotechnol. 1994; 12(10):415-20. DOI: 10.1016/0167-7799(94)90030-2. View

17.

Rapp J, Harvey A, Speksnijder G, Hu W, Ivarie R . Biologically active human interferon alpha-2b produced in the egg white of transgenic hens. Transgenic Res. 2003; 12(5):569-75. DOI: 10.1023/a:1025854217349. View

18.

Perry M . A complete culture system for the chick embryo. Nature. 1988; 331(6151):70-2. DOI: 10.1038/331070a0. View

19.

Mozdziak P, Borwornpinyo S, MCCOY D, Petitte J . Development of transgenic chickens expressing bacterial beta-galactosidase. Dev Dyn. 2003; 226(3):439-45. DOI: 10.1002/dvdy.10234. View

20.

Brown W, Hubbard S, Tickle C, Wilson S . The chicken as a model for large-scale analysis of vertebrate gene function. Nat Rev Genet. 2003; 4(2):87-98. DOI: 10.1038/nrg998. View