Gene Expression Alterations over Large Chromosomal Regions in Cancers Include Multiple Genes Unrelated to Malignant Progression

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2004 May 25

PMID 15155901

Citations 19

Authors

Brett G Masayesva

Patrick Ha

Elizabeth Garrett-Mayer

Thomas Pilkington

Rong Mao

Jonathan Pevsner

Traci Speed

Nicole Benoit

Chul-So Moon

David Sidransky

William H Westra

Joseph Califano

Affiliations

Soon will be listed here.

Abstract

In solid tumors, the relationship between DNA copy number and global expression over large chromosomal regions has not been systematically explored. We used a 12,626-gene expression array analysis of head and neck squamous cell carcinoma and normal oral mucosa and annotated gene expression levels to specific chromosomal loci. Expression alterations correlated with reported data using comparative genomic hybridization. When genes with significant differences in expression between normal and malignant lesions, as defined by significance analysis of microarrays (SAM), were compared to nonsignificant genes, similar chromosomal patterns of alteration in expression were noted. Individual tumors underwent microsatellite analysis and chi(2) analysis of expression at 3p and 22q. Significant 3p underexpression and 22q overexpression were found in all primary tumors with 3p and 22q allelic imbalance, respectively, whereas no tumor without allelic imbalance on these chromosomal arms demonstrated expression differences. Loss and gain of chromosomal material in solid cancers can alter gene expression over large chromosomal regions, including multiple genes unrelated to malignant progression.

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