New Thioredoxin Targets in the Unicellular Photosynthetic Eukaryote Chlamydomonas Reinhardtii

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2004 May 5

PMID 15123830

Citations 77

Authors

Stephane D Lemaire

Blanche Guillon

Pierre Le Marechal

Eliane Keryer

Myroslawa Miginiac-Maslow

Paulette Decottignies

Affiliations

Soon will be listed here.

Abstract

Proteomics were used to identify the proteins from the eukaryotic unicellular green alga Chlamydomonas reinhardtii that can be reduced by thioredoxin. These proteins were retained specifically on a thioredoxin affinity column made of a monocysteinic thioredoxin mutant able to form mixed disulfides with its targets. Of a total of 55 identified targets, 29 had been found previously in higher plants or Synechocystis, but 26 were new targets. Biochemical tests were performed on three of them, showing a thioredoxin-dependent activation of isocitrate lyase and isopropylmalate dehydrogenase and a thioredoxin-dependent deactivation of catalase that is redox insensitive in Arabidopsis. In addition, we identified a Ran protein, a previously uncharacterized nuclear target in a photosynthetic organism. The metabolic and evolutionary implications of these findings are discussed.

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