Studies on the Nonmevalonate Terpene Biosynthetic Pathway: Metabolic Role of IspH (LytB) Protein

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2002 Jan 31

PMID 11818558

Citations 104

Authors

Felix Rohdich

Stefan Hecht

Katrin Gartner

Petra Adam

Cornelia Krieger

Sabine Amslinger

Duilio Arigoni

Adelbert Bacher

Wolfgang Eisenreich

Affiliations

Soon will be listed here.

Abstract

Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-D-xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier in vivo experiments with recombinant Escherichia coli strains showed that exogenous 1-deoxy-D-xylulose can be converted into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate by the consecutive action of enzymes specified by the xylB and ispCDEFG genes. This article describes the transformation of exogenous [U-(13)C(5)]1-deoxy-D-xylulose into a 5:1 mixture of [U-(13)C(5)]isopentenyl diphosphate and [U-(13)C(5)]dimethylallyl diphosphate by an E. coli strain engineered for the expression of the ispH (lytB) gene in addition to recombinant xylB and ispCDEFG genes.

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