Tomato Heat Stress Protein Hsp16.1-CIII Represents a Member of a New Class of Nucleocytoplasmic Small Heat Stress Proteins in Plants

Overview

Journal Cell Stress Chaperones

Publisher Elsevier

Specialty Cell Biology

Date 2004 Apr 30

PMID 15115290

Citations 13

Authors

Masood Siddique

Markus Port

Joanna Tripp

Christian Weber

Dirk Zielinski

Raffaella Calligaris

Sibylle Winkelhaus

Klaus-Dieter Scharf

Affiliations

Soon will be listed here.

Abstract

We describe a new class of plant small heat stress proteins (sHsps) with dominant nuclear localization (Hsp17-CIII). The corresponding proteins in tomato, Arabidopsis, and rice are encoded by unique genes containing a short intron in the beta4-encoding region of the alpha-crystallin domain (ACD). The strong nuclear localization results from a cluster of basic amino acid residues in the loop between beta5 and beta6 of the ACD. Using yeast 2-hybrid tests, analyses of native complexes of the sHsps, and immunofluorescence data, we demonstrate that, in contrast to earlier observations (Kirschner et al 2000), proteins of the sHsp classes CI, CII, and CIII interact with each other, thereby influencing oligomerization state and intracellular localization.

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