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Genetic Transformation of Norway Spruce (Picea Abies (L.) Karst) Using Somatic Embryo Explants by Microprojectile Bombardment

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Journal Plant Mol Biol
Date 1992 Sep 1
PMID 1511138
Citations 9
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Abstract

Stable transformation of Norway spruce tissue has been obtained following bombardment of mature somatic embryos with pRT99gus, a plasmid that contains neo coding for NPTII, and gusA, coding for beta-glucuronidase, both fused to the CaMV 35S promoter. At least 8 lines have been stably transformed (over 15 months in culture) following bombardment and selection on kanamycin. Polymerase chain reaction analyses showed a high frequency of cotransformation of the gusA and neo genes. The frequency of coexpression of the selected and unselected markers was 100%. DNA/DNA hybridization of one transformed line provided conclusive evidence of stable integration and showed copy numbers of over 10 plasmid sequences per genome. None of the transformed lines has remained embryogenic.

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References
1.
Stomp A, Weissinger A, Sederoff R . Transient expression from microprojectile-mediated DNA transfer in pinus taeda. Plant Cell Rep. 2013; 10(4):187-90. DOI: 10.1007/BF00234292. View

2.
Spencer T, Gordon-Kamm W, Daines R, Start W, Lemaux P . Bialaphos selection of stable transformants from maize cell culture. Theor Appl Genet. 2013; 79(5):625-31. DOI: 10.1007/BF00226875. View

3.
Stomp A, Loopstra C, Chilton W, Sederoff R, Moore L . Extended Host Range of Agrobacterium tumefaciens in the Genus Pinus. Plant Physiol. 1990; 92(4):1226-32. PMC: 1062440. DOI: 10.1104/pp.92.4.1226. View

4.
Marcotte Jr W, Russell S, Quatrano R . Abscisic acid-responsive sequences from the em gene of wheat. Plant Cell. 1989; 1(10):969-76. PMC: 159833. DOI: 10.1105/tpc.1.10.969. View

5.
Gordon-Kamm W, Spencer T, Mangano M, Adams T, Daines R, Start W . Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants. Plant Cell. 1990; 2(7):603-618. PMC: 159915. DOI: 10.1105/tpc.2.7.603. View