Genetic Transformation of Maize Cells by Particle Bombardment

Overview

Journal Plant Physiol

Specialty Physiology

Date 1989 Sep 1

PMID 16667039

Citations 42

Authors

T M Klein

L Kornstein

J C Sanford

M E Fromm

Affiliations

Soon will be listed here.

Abstract

Intact maize cells were bombarded with microprojectiles bearing plasmid DNA coding for selectable (neomycin phosphotransferase [NPT II]) and screenable (beta-glucuronidase [GUS]) marker genes. Kanamycin-resistant calli were selected from bombarded cells, and these calli carried copies of the NPT II and GUS genes as determined by Southern blot analysis. All such calli expressed GUS although the level of expression varied greatly between transformed cell lines. These results show that intact cells of important monocot species can be stably transformed by microprojectiles.

Citing Articles

Unintended effects of transgenic rice revealed by transcriptome and metabolism.

Fu W, Wang C, Xu W, Zhu P, Lu Y, Wei S GM Crops Food. 2019; 10(1):20-34.

PMID: 30955410 PMC: 6592643. DOI: 10.1080/21645698.2019.1598215.

Genome-Scale Sequence Disruption Following Biolistic Transformation in Rice and Maize.

Liu J, Nannas N, Fu F, Shi J, Aspinwall B, Parrott W Plant Cell. 2019; 31(2):368-383.

PMID: 30651345 PMC: 6447018. DOI: 10.1105/tpc.18.00613.

Infection of Embryonic Callus with Enables High-Speed Transformation of Maize.

Du D, Jin R, Guo J, Zhang F Int J Mol Sci. 2019; 20(2).

PMID: 30641963 PMC: 6358778. DOI: 10.3390/ijms20020279.

A bioinformatics approach for identifying transgene insertion sites using whole genome sequencing data.

Park D, Park S, Ban Y, Kim Y, Park K, Kim N BMC Biotechnol. 2017; 17(1):67.

PMID: 28810845 PMC: 5558722. DOI: 10.1186/s12896-017-0386-x.

Genetic engineering of grass cell wall polysaccharides for biorefining.

Bhatia R, Gallagher J, Gomez L, Bosch M Plant Biotechnol J. 2017; 15(9):1071-1092.

PMID: 28557198 PMC: 5552484. DOI: 10.1111/pbi.12764.

References

Callis J, Fromm M, Walbot V . Introns increase gene expression in cultured maize cells. Genes Dev. 1987; 1(10):1183-200. DOI: 10.1101/gad.1.10.1183. View

Christou P, McCabe D, Swain W . Stable Transformation of Soybean Callus by DNA-Coated Gold Particles. Plant Physiol. 1988; 87(3):671-4. PMC: 1054818. DOI: 10.1104/pp.87.3.671. View

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

Cocking E, Davey M . Gene transfer in cereals. Science. 1987; 236(4806):1259-62. DOI: 10.1126/science.236.4806.1259. View

Koren G, Lau A, Klein J, Golas C, Soldin S, MacLeod S . Pharmacokinetics and adverse effects of amphotericin B in infants and children. J Pediatr. 1988; 113(3):559-63. DOI: 10.1016/s0022-3476(88)80653-x. View

Fromm M, Taylor L, Walbot V . Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci U S A. 1985; 82(17):5824-8. PMC: 390645. DOI: 10.1073/pnas.82.17.5824. View

Jefferson R, Kavanagh T, Bevan M . GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987; 6(13):3901-7. PMC: 553867. DOI: 10.1002/j.1460-2075.1987.tb02730.x. View

Klein T, Fromm M, Weissinger A, Tomes D, Schaaf S, Sletten M . Transfer of foreign genes into intact maize cells with high-velocity microprojectiles. Proc Natl Acad Sci U S A. 1988; 85(12):4305-9. PMC: 280417. DOI: 10.1073/pnas.85.12.4305. View

Dennis E, Gerlach W, Pryor A, Bennetzen J, Inglis A, Llewellyn D . Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res. 1984; 12(9):3983-4000. PMC: 318805. DOI: 10.1093/nar/12.9.3983. View

10.

Klein T, HARPER E, Svab Z, Sanford J, Fromm M, Maliga P . Stable genetic transformation of intact Nicotiana cells by the particle bombardment process. Proc Natl Acad Sci U S A. 1988; 85(22):8502-5. PMC: 282486. DOI: 10.1073/pnas.85.22.8502. View

11.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

12.

Fromm M, Taylor L, Walbot V . Stable transformation of maize after gene transfer by electroporation. Nature. 1986; 319(6056):791-3. DOI: 10.1038/319791a0. View

13.

Rhodes C, Pierce D, Mettler I, Mascarenhas D, Detmer J . Genetically transformed maize plants from protoplasts. Science. 1988; 240(4849):204-7. DOI: 10.1126/science.2832947. View

14.

Jefferson R, Burgess S, Hirsh D . beta-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci U S A. 1986; 83(22):8447-51. PMC: 386947. DOI: 10.1073/pnas.83.22.8447. View

15.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View