PerlPrimer: Cross-platform, Graphical Primer Design for Standard, Bisulphite and Real-time PCR

Overview

Journal Bioinformatics

Publisher Oxford University Press

Specialty Biology

Date 2004 Apr 10

PMID 15073005

Citations 251

Authors

Owen J Marshall

Affiliations

Soon will be listed here.

Abstract

Unlabelled: PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer-dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design.

Availability: Open-source and freely available from http://perlprimer.sourceforge.net.

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