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PerlPrimer: Cross-platform, Graphical Primer Design for Standard, Bisulphite and Real-time PCR

Overview
Journal Bioinformatics
Specialty Biology
Date 2004 Apr 10
PMID 15073005
Citations 251
Authors
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Abstract

Unlabelled: PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer-dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design.

Availability: Open-source and freely available from http://perlprimer.sourceforge.net.

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