Protein-protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2004 Apr 6

PMID 15064450

Citations 17

Authors

Guanghua Gao

Jason G Williams

Sharon L Campbell

Affiliations

Soon will be listed here.

Abstract

Nuclear magnetic resonance (NMR) is a powerful technique to study protein-protein interactions in solution. Various methods have been developed and applied successfully for locating binding sites on proteins. The application of NMR chemical-shift perturbation to map the protein-protein interfaces is described in this chapter, providing a practical guideline that can be followed to carry out the experiments.

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