Conventional PKCs Regulate the Temporal Pattern of Ca2+ Oscillations at Fertilization in Mouse Eggs

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2004 Mar 31

PMID 15051735

Citations 18

Authors

Guillaume Halet

Richard Tunwell

Scott J Parkinson

John Carroll

Affiliations

Soon will be listed here.

Abstract

In mammalian eggs, sperm-induced Ca2+ oscillations at fertilization are the primary trigger for egg activation and initiation of embryonic development. Identifying the downstream effectors that decode this unique Ca2+ signal is essential to understand how the transition from egg to embryo is coordinated. Here, we investigated whether conventional PKCs (cPKCs) can decode Ca2+ oscillations at fertilization. By monitoring the dynamics of GFP-labeled PKCalpha and PKCgamma in living mouse eggs, we demonstrate that cPKCs translocate to the egg membrane at fertilization following a pattern that is shaped by the amplitude, duration, and frequency of the Ca2+ transients. In addition, we show that cPKC translocation is driven by the C2 domain when Ca2+ concentration reaches 1-3 microM. Finally, we present evidence that one physiological function of activated cPKCs in fertilized eggs is to sustain long-lasting Ca2+ oscillations, presumably via the regulation of store-operated Ca2+ entry.

Citing Articles

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