» Articles » PMID: 14970190

Amylin Inhibits Bone Resorption While the Calcitonin Receptor Controls Bone Formation in Vivo

Overview
Journal J Cell Biol
Specialty Cell Biology
Date 2004 Feb 19
PMID 14970190
Citations 54
Authors
Affiliations
Soon will be listed here.
Abstract

Amylin is a member of the calcitonin family of hormones cosecreted with insulin by pancreatic beta cells. Cell culture assays suggest that amylin could affect bone formation and bone resorption, this latter function after its binding to the calcitonin receptor (CALCR). Here we show that Amylin inactivation leads to a low bone mass due to an increase in bone resorption, whereas bone formation is unaffected. In vitro, amylin inhibits fusion of mononucleated osteoclast precursors into multinucleated osteoclasts in an ERK1/2-dependent manner. Although Amylin +/- mice like Amylin-deficient mice display a low bone mass phenotype and increased bone resorption, Calcr +/- mice display a high bone mass due to an increase in bone formation. Moreover, compound heterozygote mice for Calcr and Amylin inactivation displayed bone abnormalities observed in both Calcr +/- and Amylin +/- mice, thereby ruling out that amylin uses CALCR to inhibit osteoclastogenesis in vivo. Thus, amylin is a physiological regulator of bone resorption that acts through an unidentified receptor.

Citing Articles

The role of amylin, a gut-brain axis hormone, in metabolic and neurological disorders.

Muhammad T, Pastore S, Good K, Yu W, Vincent J FASEB Bioadv. 2025; 7(3):e1480.

PMID: 40060942 PMC: 11886606. DOI: 10.1096/fba.2024-00151.


Beyond resorption: osteoclasts as drivers of bone formation.

Xiang Q, Li L, Ji W, Gawlitta D, Walboomers X, van den Beucken J Cell Regen. 2024; 13(1):22.

PMID: 39392536 PMC: 11469995. DOI: 10.1186/s13619-024-00205-x.


Identification of key immune genes of osteoporosis based on bioinformatics and machine learning.

Hao S, Xinqi M, Weicheng X, Shiwei Y, Lumin C, Xiao W Front Endocrinol (Lausanne). 2023; 14:1118886.

PMID: 37361541 PMC: 10289263. DOI: 10.3389/fendo.2023.1118886.


Role of Calcr expressing neurons in the medial amygdala in social contact among females.

Fukumitsu K, Huang A, McHugh T, Kuroda K Mol Brain. 2023; 16(1):10.

PMID: 36658598 PMC: 9850531. DOI: 10.1186/s13041-023-00993-4.


Islet amyloid polypeptide does not suppress pancreatic cancer.

Taylor A, Panzhinskiy E, Orban P, Lynn F, Schaeffer D, Johnson J Mol Metab. 2023; 68:101667.

PMID: 36621763 PMC: 9938314. DOI: 10.1016/j.molmet.2023.101667.


References
1.
Zaidi M, Pazianas M, Shankar V, Bax B, Bax C, Bevis P . Osteoclast function and its control. Exp Physiol. 1993; 78(6):721-39. DOI: 10.1113/expphysiol.1993.sp003721. View

2.
Cornish J, Callon K, Lin C, Xiao C, Gamble G, Cooper G . Comparison of the effects of calcitonin gene-related peptide and amylin on osteoblasts. J Bone Miner Res. 1999; 14(8):1302-9. DOI: 10.1359/jbmr.1999.14.8.1302. View

3.
Wimalawansa S . Amylin, calcitonin gene-related peptide, calcitonin, and adrenomedullin: a peptide superfamily. Crit Rev Neurobiol. 1997; 11(2-3):167-239. DOI: 10.1615/critrevneurobiol.v11.i2-3.40. View

4.
Suda T, Jimi E, Nakamura I, Takahashi N . Role of 1 alpha,25-dihydroxyvitamin D3 in osteoclast differentiation and function. Methods Enzymol. 1997; 282:223-35. DOI: 10.1016/s0076-6879(97)82110-6. View

5.
Anusaksathien O, Laplace C, Li X, Ren Y, Peng L, Goldring S . Tissue-specific and ubiquitous promoters direct the expression of alternatively spliced transcripts from the calcitonin receptor gene. J Biol Chem. 2001; 276(25):22663-74. DOI: 10.1074/jbc.M007104200. View