Identification of Androgen-coregulated Protein Networks from the Microsomes of Human Prostate Cancer Cells

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2004 Jan 8

PMID 14709176

Citations 11

Authors

Michael E Wright

Jimmy Eng

James Sherman

David M Hockenbery

Peter S Nelson

Timothy Galitski

Ruedi Aebersold

Affiliations

Soon will be listed here.

Abstract

Background: Androgens play a critical role in the development of prostate cancer-dysregulation of androgen-regulated growth pathways can led to hormone-refractory prostate cancer. A comprehensive understanding of androgen-regulated cellular processes has not been achieved to date. To this end, we have applied a large-scale proteomic approach to define cellular processes that are responsive to androgen treatment in LNCaP prostate cancer cells.

Results: Using isotope-coded affinity tags and mass spectrometry we identified and quantified the relative abundance levels of 1,064 proteins and found that distinct cellular processes were coregulated by androgen while others were essentially unaffected. Subsequent pharmacological perturbation of the cellular process for energy generation confirmed that androgen starvation had a profound effect on this pathway.

Conclusions: Our results provide evidence for the role of androgenic hormones in coordinating the expression of critical components involved in distinct cellular processes and further establish a foundation for the comprehensive reconstruction of androgen-regulated protein networks and pathways in prostate cancer cells.

Citing Articles

Endothelial Function in Postmenopausal Women: The Possible Role of Heat Shock Protein 60 and Serum Androgens.

Armeni E, Soureti A, Augoulea A, Chondrou A, Drakoulis N, Kaparos G Front Mol Med. 2024; 2:933188.

PMID: 39086968 PMC: 11285708. DOI: 10.3389/fmmed.2022.933188.

Review of the Real and Sometimes Hidden Costs in Proteomics Experimental Workflows.

Houfani A, Foster L Methods Mol Biol. 2022; 2456:1-14.

PMID: 35612731 DOI: 10.1007/978-1-0716-2124-0_1.

Research Resource: Androgen Receptor Activity Is Regulated Through the Mobilization of Cell Surface Receptor Networks.

Hsiao J, Ng B, Smits M, Martinez H, Jasavala R, Hinkson I Mol Endocrinol. 2015; 29(8):1195-218.

PMID: 26181434 PMC: 4517998. DOI: 10.1210/me.2015-1021.

Androgen receptor and chemokine receptors 4 and 7 form a signaling axis to regulate CXCL12-dependent cellular motility.

Hsiao J, Ng B, Smits M, Wang J, Jasavala R, Martinez H BMC Cancer. 2015; 15:204.

PMID: 25884570 PMC: 4393632. DOI: 10.1186/s12885-015-1201-5.

Androgen-sensitive microsomal signaling networks coupled to the proliferation and differentiation of human prostate cancer cells.

Martinez H, Hsiao J, Jasavala R, Hinkson I, Eng J, Wright M Genes Cancer. 2012; 2(10):956-78.

PMID: 22701762 PMC: 3374630. DOI: 10.1177/1947601912436422.

References

Marcelli M, Ittmann M, Mariani S, Sutherland R, Nigam R, Murthy L . Androgen receptor mutations in prostate cancer. Cancer Res. 2000; 60(4):944-9. View

Brazhnik P, de la Fuente A, Mendes P . Gene networks: how to put the function in genomics. Trends Biotechnol. 2002; 20(11):467-72. DOI: 10.1016/s0167-7799(02)02053-x. View

Ferdinandusse S, Denis S, IJlst L, dAcremont G, Waterham H, Wanders R . Subcellular localization and physiological role of alpha-methylacyl-CoA racemase. J Lipid Res. 2000; 41(11):1890-6. View

Costello L, Franklin R . The intermediary metabolism of the prostate: a key to understanding the pathogenesis and progression of prostate malignancy. Oncology. 2000; 59(4):269-82. PMC: 4472372. DOI: 10.1159/000012183. View

Dye B, Patton J . An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles. Exp Cell Res. 2001; 263(1):131-44. DOI: 10.1006/excr.2000.5097. View

Lander E, Linton L, Birren B, Nusbaum C, Zody M, Baldwin J . Initial sequencing and analysis of the human genome. Nature. 2001; 409(6822):860-921. DOI: 10.1038/35057062. View

Muller J, Isele U, Metzger E, Rempel A, Moser M, Pscherer A . FHL2, a novel tissue-specific coactivator of the androgen receptor. EMBO J. 2000; 19(3):359-69. PMC: 305573. DOI: 10.1093/emboj/19.3.359. View

Afar D, Vivanco I, Hubert R, Kuo J, Chen E, Saffran D . Catalytic cleavage of the androgen-regulated TMPRSS2 protease results in its secretion by prostate and prostate cancer epithelia. Cancer Res. 2001; 61(4):1686-92. View

Waghray A, Schober M, Feroze F, Yao F, Virgin J, Chen Y . Identification of differentially expressed genes by serial analysis of gene expression in human prostate cancer. Cancer Res. 2001; 61(10):4283-6. View

10.

Grabowski P, Black D . Alternative RNA splicing in the nervous system. Prog Neurobiol. 2001; 65(3):289-308. DOI: 10.1016/s0301-0082(01)00007-7. View

11.

Han D, Eng J, Zhou H, Aebersold R . Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry. Nat Biotechnol. 2001; 19(10):946-51. PMC: 1444949. DOI: 10.1038/nbt1001-946. View

12.

Jiang Z, Woda B, Rock K, Xu Y, Savas L, Khan A . P504S: a new molecular marker for the detection of prostate carcinoma. Am J Surg Pathol. 2001; 25(11):1397-404. DOI: 10.1097/00000478-200111000-00007. View

13.

Waghray A, Feroze F, Schober M, Yao F, Wood C, Puravs E . Identification of androgen-regulated genes in the prostate cancer cell line LNCaP by serial analysis of gene expression and proteomic analysis. Proteomics. 2001; 1(10):1327-38. DOI: 10.1002/1615-9861(200110)1:10<1327::AID-PROT1327>3.0.CO;2-B. View

14.

Yang F, Li X, Sharma M, Sasaki C, Longo D, Lim B . Linking beta-catenin to androgen-signaling pathway. J Biol Chem. 2002; 277(13):11336-44. DOI: 10.1074/jbc.M111962200. View

15.

Rubin M, Zhou M, Dhanasekaran S, Varambally S, Barrette T, Sanda M . alpha-Methylacyl coenzyme A racemase as a tissue biomarker for prostate cancer. JAMA. 2002; 287(13):1662-70. DOI: 10.1001/jama.287.13.1662. View

16.

Luo J, Zha S, Gage W, Dunn T, Hicks J, Bennett C . Alpha-methylacyl-CoA racemase: a new molecular marker for prostate cancer. Cancer Res. 2002; 62(8):2220-6. View

17.

Pawlowski J, Ertel J, Allen M, Xu M, Butler C, Wilson E . Liganded androgen receptor interaction with beta-catenin: nuclear co-localization and modulation of transcriptional activity in neuronal cells. J Biol Chem. 2002; 277(23):20702-10. DOI: 10.1074/jbc.M200545200. View

18.

Zhou M, Chinnaiyan A, Kleer C, Lucas P, Rubin M . Alpha-Methylacyl-CoA racemase: a novel tumor marker over-expressed in several human cancers and their precursor lesions. Am J Surg Pathol. 2002; 26(7):926-31. DOI: 10.1097/00000478-200207000-00012. View

19.

Smith J, Marelli M, Christmas R, Vizeacoumar F, Dilworth D, Ideker T . Transcriptome profiling to identify genes involved in peroxisome assembly and function. J Cell Biol. 2002; 158(2):259-71. PMC: 2173120. DOI: 10.1083/jcb.200204059. View

20.

Ashburner M, Lewis S . On ontologies for biologists: the Gene Ontology--untangling the web. Novartis Found Symp. 2003; 247:66-80; discussion 80-3, 84-90, 244-52. View