Ligase-mediated Construction of Branched DNA Strands: a Novel DNA Joining Activity Catalyzed by T4 DNA Ligase

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2004 Jan 6

PMID 14704359

Citations 7

Authors

Maritha Mendel-Hartvig

Anil Kumar

Ulf Landegren

Affiliations

Soon will be listed here.

Abstract

Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here how T4 DNA ligase can be used for efficient construction of DNA molecules having one 5' end but two distinct 3' ends that extend from the 2' and 3' carbons, respectively, of an internal nucleotide. The nature of the reaction products is investigated, and optimal reaction conditions are reported for the construction of branched oligonucleotides. We discuss the utility of these branched DNA nanostructures for gene detection.

Citing Articles

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Dual coding potential of a 2',5'-branched ribonucleotide in DNA.

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Theoretical Analysis of a Self-Replicator With Reduced Template Inhibition Based on an Informational Leaving Group.

Bigan E, Mattelaer H, Herdewijn P J Mol Evol. 2016; 82(2-3):93-109.

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DNA ligase-mediated translation of DNA into densely functionalized nucleic acid polymers.

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