Nuclear Localization of Avian Polyomavirus Structural Protein VP1 is a Prerequisite for the Formation of Virus-like Particles

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2003 Dec 25

PMID 14694124

Citations 3

Authors

Reimar Johne

Hermann Muller

Affiliations

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Abstract

Virions of polyomaviruses consist of the major structural protein VP1, the minor structural proteins VP2 and VP3, and the viral genome associated with histones. An additional structural protein, VP4, is present in avian polyomavirus (APV) particles. As it had been reported that expression of APV VP1 in insect cells did not result in the formation of virus-like particles (VLP), the prerequisites for particle formation were analyzed. To this end, recombinant influenza viruses were created to (co)express the structural proteins of APV in chicken embryo cells, permissive for APV replication. VP1 expressed individually or coexpressed with VP4 did not result in VLP formation; both proteins (co)localized in the cytoplasm. Transport of VP1, or the VP1-VP4 complex, into the nucleus was facilitated by the coexpression of VP3 and resulted in the formation of VLP. Accordingly, a mutant APV VP1 carrying the N-terminal nuclear localization signal of simian virus 40 VP1 was transported to the nucleus and assembled into VLP. These results support a model of APV capsid assembly in which complexes of the structural proteins VP1, VP3 (or VP2), and VP4, formed within the cytoplasm, are transported to the nucleus using the nuclear localization signal of VP3 (or VP2); there, capsid formation is induced by the nuclear environment.

Citing Articles

The structure of avian polyomavirus reveals variably sized capsids, non-conserved inter-capsomere interactions, and a possible location of the minor capsid protein VP4.

Shen P, Enderlein D, Nelson C, Carter W, Kawano M, Xing L Virology. 2011; 411(1):142-52.

PMID: 21239031 PMC: 3057058. DOI: 10.1016/j.virol.2010.12.005.

Polyomaviruses of birds: etiologic agents of inflammatory diseases in a tumor virus family.

Johne R, Muller H J Virol. 2007; 81(21):11554-9.

PMID: 17715213 PMC: 2168798. DOI: 10.1128/JVI.01178-07.

Characterization of two novel polyomaviruses of birds by using multiply primed rolling-circle amplification of their genomes.

Johne R, Wittig W, Fernandez-de-Luco D, Hofle U, Muller H J Virol. 2006; 80(7):3523-31.

PMID: 16537620 PMC: 1440385. DOI: 10.1128/JVI.80.7.3523-3531.2006.

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