Mechanism of Assembly of Recombinant Murine Polyomavirus-like Particles

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2000 Jan 22

PMID 10644335

Citations 8

Authors

U Schmidt

R Rudolph

G Bohm

Affiliations

Soon will be listed here.

Abstract

VP1 is the major viral coat protein of murine polyomavirus and can be used for the generation of virus-like particles in vitro. Here, we demonstrate that capsid assembly is an equilibrium reaction followed by oxidation of intracapsomere disulfide bonds, which are not essential for the formation of virus-like particles but enable complete particle assembly and prevent capsid disassembly.

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References

Garcea R, Salunke D, Caspar D . Site-directed mutation affecting polyomavirus capsid self-assembly in vitro. Nature. 1987; 329(6134):86-7. DOI: 10.1038/329086a0. View

Schmidt U, Kenklies J, Rudolph R, Bohm G . Site-specific fluorescence labelling of recombinant polyomavirus-like particles. Biol Chem. 1999; 380(3):397-401. DOI: 10.1515/BC.1999.053. View

Liddington R, Yan Y, Moulai J, Sahli R, BENJAMIN T, Harrison S . Structure of simian virus 40 at 3.8-A resolution. Nature. 1991; 354(6351):278-84. DOI: 10.1038/354278a0. View

Stehle T, Yan Y, BENJAMIN T, Harrison S . Structure of murine polyomavirus complexed with an oligosaccharide receptor fragment. Nature. 1994; 369(6476):160-3. DOI: 10.1038/369160a0. View

Chong S, Shao Y, Paulus H, Benner J, Perler F, Xu M . Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system. J Biol Chem. 1996; 271(36):22159-68. DOI: 10.1074/jbc.271.36.22159. View

Stehle T, Gamblin S, Yan Y, Harrison S . The structure of simian virus 40 refined at 3.1 A resolution. Structure. 1996; 4(2):165-82. DOI: 10.1016/s0969-2126(96)00020-2. View

Stehle T, Harrison S . Crystal structures of murine polyomavirus in complex with straight-chain and branched-chain sialyloligosaccharide receptor fragments. Structure. 1996; 4(2):183-94. DOI: 10.1016/s0969-2126(96)00021-4. View

Chong S, Xu M . Protein splicing of the Saccharomyces cerevisiae VMA intein without the endonuclease motifs. J Biol Chem. 1997; 272(25):15587-90. DOI: 10.1074/jbc.272.25.15587. View

Mackay R, Consigli R . Early events in polyoma virus infection: attachment, penetration, and nuclear entry. J Virol. 1976; 19(2):620-36. PMC: 354897. DOI: 10.1128/JVI.19.2.620-636.1976. View

10.

Christiansen G, Landers T, Griffith J, Berg P . Characterization of components released by alkali disruption of simian virus 40. J Virol. 1977; 21(3):1079-84. PMC: 515648. DOI: 10.1128/JVI.21.3.1079-1084.1977. View

11.

Brady J, Winston V, Consigli R . Dissociation of polyoma virus by the chelation of calcium ions found associated with purified virions. J Virol. 1977; 23(3):717-24. PMC: 515884. DOI: 10.1128/JVI.23.3.717-724.1977. View

12.

Brady J, Winston V, Consigli R . Characterization of a DNA-protein complex and capsomere subunits derived from polyoma virus by treatment with ethyleneglycol-bis-N,N'-tetraacetic acid and dithiothreitol. J Virol. 1978; 27(1):193-204. PMC: 354152. DOI: 10.1128/JVI.27.1.193-204.1978. View

13.

Leavitt A, Roberts T, Garcea R . Polyoma virus major capsid protein, VP1. Purification after high level expression in Escherichia coli. J Biol Chem. 1985; 260(23):12803-9. View

14.

Salunke D, Caspar D, Garcea R . Self-assembly of purified polyomavirus capsid protein VP1. Cell. 1986; 46(6):895-904. DOI: 10.1016/0092-8674(86)90071-1. View

15.

Chong S, Mersha F, COMB D, Scott M, Landry D, Vence L . Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene. 1997; 192(2):271-81. DOI: 10.1016/s0378-1119(97)00105-4. View

16.

Li M, Beard P, Estes P, Lyon M, Garcea R . Intercapsomeric disulfide bonds in papillomavirus assembly and disassembly. J Virol. 1998; 72(3):2160-7. PMC: 109511. DOI: 10.1128/JVI.72.3.2160-2167.1998. View

17.

Chong S, Williams K, Wotkowicz C, Xu M . Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein. J Biol Chem. 1998; 273(17):10567-77. DOI: 10.1074/jbc.273.17.10567. View

18.

Braun H, Boller K, Lower J, Bertling W, Zimmer A . Oligonucleotide and plasmid DNA packaging into polyoma VP1 virus-like particles expressed in Escherichia coli. Biotechnol Appl Biochem. 1999; 29 ( Pt 1):31-43. View

19.

Salunke D, Caspar D, Garcea R . Polymorphism in the assembly of polyomavirus capsid protein VP1. Biophys J. 1989; 56(5):887-900. PMC: 1280588. DOI: 10.1016/S0006-3495(89)82735-3. View