Direct Observation of Tiers in the Energy Landscape of a Chromoprotein: a Single-molecule Study

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2003 Dec 13

PMID 14671325

Citations 34

Authors

Clemens Hofmann

Thijs J Aartsma

Hartmut Michel

Jurgen Kohler

Affiliations

Soon will be listed here.

Abstract

Single-molecule spectroscopic techniques were applied to individual pigments embedded in a chromoprotein. A sensitive tool to monitor structural fluctuations of the protein backbone in the local environment of the chromophore is provided by recording the changes of the spectral positions of the pigment absorptions as a function of time. The data provide information about the organization of the energy landscape of the protein in tiers that can be characterized by an average barrier height. Additionally, a correlation between the average barrier height within a distinct tier and the time scale of the structural fluctuations is observed.

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