Variation of Exciton-vibrational Coupling in Photosystem II Core Complexes from Thermosynechococcus Elongatus As Revealed by Single-molecule Spectroscopy

Overview

Journal J Phys Chem B

Publisher American Chemical Society

Specialty Chemistry

Date 2015 Feb 25

PMID 25708355

Citations 1

Authors

Sepideh Skandary

Martin Hussels

Alexander Konrad

Thomas Renger

Frank Muh

Martin Bommer

Athina Zouni

Alfred J Meixner

Marc Brecht

Affiliations

Soon will be listed here.

Abstract

The spectral properties and dynamics of the fluorescence emission of photosystem II core complexes are investigated by single-molecule spectroscopy at 1.6 K. The emission spectra are dominated by sharp zero-phonon lines (ZPLs). The sharp ZPLs are the result of weak to intermediate exciton-vibrational coupling and slow spectral diffusion. For several data sets, it is possible to surpass the effect of spectral diffusion by applying a shifting algorithm. The increased signal-to-noise ratio enables us to determine the exciton-vibrational coupling strength (Huang-Rhys factor) with high precision. The Huang-Rhys factors vary between 0.03 and 0.8. The values of the Huang-Rhys factors show no obvious correlation between coupling strength and wavelength position. From this result, we conclude that electrostatic rather than exchange or dispersive interactions are the main contributors to the exciton-vibrational coupling in this system.

Citing Articles

Structural basis of light-harvesting in the photosystem II core complex.

Muh F, Zouni A Protein Sci. 2020; 29(5):1090-1119.

PMID: 32067287 PMC: 7184784. DOI: 10.1002/pro.3841.

References

Shibata Y, Nishi S, Kawakami K, Shen J, Renger T . Photosystem II does not possess a simple excitation energy funnel: time-resolved fluorescence spectroscopy meets theory. J Am Chem Soc. 2013; 135(18):6903-14. PMC: 3650659. DOI: 10.1021/ja312586p. View

Komura M, Shibata Y, Itoh S . A new fluorescence band F689 in photosystem II revealed by picosecond analysis at 4-77 K: function of two terminal energy sinks F689 and F695 in PS II. Biochim Biophys Acta. 2006; 1757(12):1657-68. DOI: 10.1016/j.bbabio.2006.09.007. View

Brecht M, Skandary S, Hellmich J, Glockner C, Konrad A, Hussels M . Spectroscopic properties of photosystem II core complexes from Thermosynechococcus elongatus revealed by single-molecule experiments. Biochim Biophys Acta. 2014; 1837(6):773-81. DOI: 10.1016/j.bbabio.2014.01.025. View

Kunz R, Timpmann K, Southall J, Cogdell R, Freiberg A, Kohler J . Exciton self trapping in photosynthetic pigment-protein complexes studied by single-molecule spectroscopy. J Phys Chem B. 2012; 116(36):11017-23. DOI: 10.1021/jp3040456. View

Caffarri S, Kouril R, Kereiche S, Boekema E, Croce R . Functional architecture of higher plant photosystem II supercomplexes. EMBO J. 2009; 28(19):3052-63. PMC: 2760109. DOI: 10.1038/emboj.2009.232. View

Hofmann C, Ketelaars M, Matsushita M, Michel H, Aartsma T, Kohler J . Single-molecule study of the electronic couplings in a circular array of molecules: light-harvesting-2 complex from Rhodospirillum molischianum. Phys Rev Lett. 2003; 90(1):013004. DOI: 10.1103/PhysRevLett.90.013004. View

Renger T, Schlodder E . Optical properties, excitation energy and primary charge transfer in photosystem II: theory meets experiment. J Photochem Photobiol B. 2011; 104(1-2):126-41. DOI: 10.1016/j.jphotobiol.2011.03.016. View

Reppert M, Zazubovich V, Dang N, Seibert M, Jankowiak R . Low-energy chlorophyll states in the CP43 antenna protein complex: simulation of various optical spectra. II. J Phys Chem B. 2008; 112(32):9934-47. DOI: 10.1021/jp8013749. View

Hofmann C, Michel H, van Heel M, Kohler J . Multivariate analysis of single-molecule spectra: surpassing spectral diffusion. Phys Rev Lett. 2005; 94(19):195501. DOI: 10.1103/PhysRevLett.94.195501. View

10.

Scholes G, Fleming G, Olaya-Castro A, van Grondelle R . Lessons from nature about solar light harvesting. Nat Chem. 2011; 3(10):763-74. DOI: 10.1038/nchem.1145. View

11.

Muh F, Zouni A . Light-induced water oxidation in photosystem II. Front Biosci (Landmark Ed). 2011; 16(8):3072-132. DOI: 10.2741/3900. View

12.

Muh F, Madjet M, Renger T . Structure-based simulation of linear optical spectra of the CP43 core antenna of photosystem II. Photosynth Res. 2011; 111(1-2):87-101. DOI: 10.1007/s11120-011-9675-8. View

13.

Umena Y, Kawakami K, Shen J, Kamiya N . Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å. Nature. 2011; 473(7345):55-60. DOI: 10.1038/nature09913. View

14.

Brecht M, Studier H, Radics V, Nieder J, Bittl R . Spectral diffusion induced by proton dynamics in pigment-protein complexes. J Am Chem Soc. 2008; 130(51):17487-93. DOI: 10.1021/ja806216p. View

15.

Loll B, Kern J, Saenger W, Zouni A, Biesiadka J . Towards complete cofactor arrangement in the 3.0 A resolution structure of photosystem II. Nature. 2005; 438(7070):1040-4. DOI: 10.1038/nature04224. View

16.

Renger T . Theory of optical spectra involving charge transfer states: dynamic localization predicts a temperature dependent optical band shift. Phys Rev Lett. 2004; 93(18):188101. DOI: 10.1103/PhysRevLett.93.188101. View

17.

Najafi M, Herascu N, Seibert M, Picorel R, Jankowiak R, Zazubovich V . Spectral hole burning, recovery, and thermocycling in chlorophyll-protein complexes: distributions of barriers on the protein energy landscape. J Phys Chem B. 2012; 116(38):11780-90. DOI: 10.1021/jp308055r. View

18.

Rutkauskas D, Novoderezhkin V, Cogdell R, van Grondelle R . Fluorescence spectroscopy of conformational changes of single LH2 complexes. Biophys J. 2004; 88(1):422-35. PMC: 1305019. DOI: 10.1529/biophysj.104.048629. View

19.

Muh F, Glockner C, Hellmich J, Zouni A . Light-induced quinone reduction in photosystem II. Biochim Biophys Acta. 2011; 1817(1):44-65. DOI: 10.1016/j.bbabio.2011.05.021. View

20.

Herascu N, Najafi M, Amunts A, Pieper J, Irrgang K, Picorel R . Parameters of the protein energy landscapes of several light-harvesting complexes probed via spectral hole growth kinetics measurements. J Phys Chem B. 2011; 115(12):2737-47. DOI: 10.1021/jp108775y. View