Chromosome-protein Interactions in Polyomavirus Virions

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2003 Dec 13

PMID 14671132

Citations 3

Authors

Mariarosaria Carbone

Giuseppe Ascione

Silvia Chichiarelli

Marie-Isabelle Garcia

Margherita Eufemi

Paolo Amati

Affiliations

Soon will be listed here.

Abstract

In this work, we sought to determine whether the components of the murine polyomavirus capsid establish specific interactions with the minichromosome encapsidated into the mature viral particles by using the cis-diamminedichloroplatinum(II) cross-linking reagent. Our data indicated that VP1, but not minor capsid proteins, interacts with the viral genome in vivo. In addition, semiquantitative PCR assays performed on cross-linked DNA complexes revealed that VP1 binds to all regions of the viral genome but significantly more to the regulatory region. The implications of such an interaction for viral infectivity are discussed.

Citing Articles

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A polyomavirus peptide binds to the capsid VP1 pore and has potent antiviral activity against BK and JC polyomaviruses.

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