Membrane Enzymes: Artifacts in Arrhenius Plots Due to Temperature Dependence of Substrate-binding Affinity

Overview

Journal Science

Specialty Science

Date 1978 Feb 24

PMID 146257

Citations 15

Authors

J R Silvius

B D Read

R N McElhaney

Affiliations

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Abstract

For the membrane sodium-stimulated magnesium-adenosinetriphosphatase of Acholeplasma laidlawii B both the Vmax and Km values in the Michaelis equation very strongly with temperature. Simulations of Arrhenius plots show that an enzyme with a temperature-dependent Km can yield a variety of Arrhenius plot artifacts, most notably erroneous "breaks," if activity is assayed at a fixed substrate concentration.

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