Yeast Life-span Extension by Calorie Restriction is Independent of NAD Fluctuation

Overview

Journal Science

Specialty Science

Date 2003 Nov 8

PMID 14605207

Citations 60

Authors

Rozalyn M Anderson

Magda Latorre-Esteves

Ana Rute Neves

Siva Lavu

Oliver Medvedik

Christopher Taylor

Konrad T Howitz

Helena Santos

David A Sinclair

Affiliations

Soon will be listed here.

Abstract

Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.

Citing Articles

SIRT1 improves lactate homeostasis in the brain to alleviate parkinsonism via deacetylation and inhibition of PKM2.

Lian B, Zhang J, Yin X, Wang J, Li L, Ju Q Cell Rep Med. 2024; 5(8):101684.

PMID: 39128469 PMC: 11384727. DOI: 10.1016/j.xcrm.2024.101684.

A Simple, Fast, Sensitive LC-MS/MS Method to Quantify NAD(H) in Biological Samples: Plasma NAD(H) Measurement to Monitor Brain Pathophysiology.

Ishima T, Kimura N, Kobayashi M, Nagai R, Osaka H, Aizawa K Int J Mol Sci. 2024; 25(4).

PMID: 38397001 PMC: 10888655. DOI: 10.3390/ijms25042325.

Dietary change without caloric restriction maintains a youthful profile in ageing yeast.

Horkai D, Hadj-Moussa H, Whale A, Houseley J PLoS Biol. 2023; 21(8):e3002245.

PMID: 37643155 PMC: 10464975. DOI: 10.1371/journal.pbio.3002245.

Metabolic decline in an insect ear: correlative or causative for age-related auditory decline?.

Austin T, Thomas C, Lewis C, Blockley A, Warren B Front Cell Dev Biol. 2023; 11:1138392.

PMID: 37274746 PMC: 10233746. DOI: 10.3389/fcell.2023.1138392.

Recent Neurotherapeutic Strategies to Promote Healthy Brain Aging: Are we there yet?.

Kim C, Sachdev P, Braidy N Aging Dis. 2022; 13(1):175-214.

PMID: 35111369 PMC: 8782556. DOI: 10.14336/AD.2021.0705.

References

Jiang J, Jaruga E, Repnevskaya M, Jazwinski S . An intervention resembling caloric restriction prolongs life span and retards aging in yeast. FASEB J. 2000; 14(14):2135-7. DOI: 10.1096/fj.00-0242fje. View

Lin S, Guarente L . Nicotinamide adenine dinucleotide, a metabolic regulator of transcription, longevity and disease. Curr Opin Cell Biol. 2003; 15(2):241-6. DOI: 10.1016/s0955-0674(03)00006-1. View

SWIECILO A, Krawiec Z, Wawryn J, Bartosz G, Bilinski T . Effect of stress on the life span of the yeast Saccharomyces cerevisiae. Acta Biochim Pol. 2000; 47(2):355-64. View

Shama S, Lai C, Antoniazzi J, Jiang J, Jazwinski S . Heat stress-induced life span extension in yeast. Exp Cell Res. 1998; 245(2):379-88. DOI: 10.1006/excr.1998.4279. View

Zhang Q, Piston D, Goodman R . Regulation of corepressor function by nuclear NADH. Science. 2002; 295(5561):1895-7. DOI: 10.1126/science.1069300. View

Lin S, Defossez P, Guarente L . Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae. Science. 2000; 289(5487):2126-8. DOI: 10.1126/science.289.5487.2126. View

Anderson R, Bitterman K, Wood J, Medvedik O, Sinclair D . Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae. Nature. 2003; 423(6936):181-5. PMC: 4802858. DOI: 10.1038/nature01578. View

Hekimi S, Guarente L . Genetics and the specificity of the aging process. Science. 2003; 299(5611):1351-4. DOI: 10.1126/science.1082358. View

Paine P . Nucleocytoplasmic movement of fluorescent tracers microinjected into living salivary gland cells. J Cell Biol. 1975; 66(3):652-7. PMC: 2109458. DOI: 10.1083/jcb.66.3.652. View

10.

Anderson R, Bitterman K, Wood J, Medvedik O, Cohen H, Lin S . Manipulation of a nuclear NAD+ salvage pathway delays aging without altering steady-state NAD+ levels. J Biol Chem. 2002; 277(21):18881-90. DOI: 10.1074/jbc.M111773200. View

11.

Penfound T, FOSTER J . NAD-dependent DNA-binding activity of the bifunctional NadR regulator of Salmonella typhimurium. J Bacteriol. 1999; 181(2):648-55. PMC: 93422. DOI: 10.1128/JB.181.2.648-655.1999. View

12.

Fulco M, Schiltz R, Iezzi S, King M, Zhao P, Kashiwaya Y . Sir2 regulates skeletal muscle differentiation as a potential sensor of the redox state. Mol Cell. 2003; 12(1):51-62. DOI: 10.1016/s1097-2765(03)00226-0. View

13.

Imai S, ARMSTRONG C, Kaeberlein M, Guarente L . Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature. 2000; 403(6771):795-800. DOI: 10.1038/35001622. View

14.

Pahlman A, Granath K, Ansell R, Hohmann S, Adler L . The yeast glycerol 3-phosphatases Gpp1p and Gpp2p are required for glycerol biosynthesis and differentially involved in the cellular responses to osmotic, anaerobic, and oxidative stress. J Biol Chem. 2000; 276(5):3555-63. DOI: 10.1074/jbc.M007164200. View

15.

Neves A, Ventura R, Mansour N, Shearman C, Gasson M, Maycock C . Is the glycolytic flux in Lactococcus lactis primarily controlled by the redox charge? Kinetics of NAD(+) and NADH pools determined in vivo by 13C NMR. J Biol Chem. 2002; 277(31):28088-98. DOI: 10.1074/jbc.M202573200. View

16.

Unkefer C, London R . In vivo studies of pyridine nucleotide metabolism in Escherichia coli and Saccharomyces cerevisiae by carbon-13 NMR spectroscopy. J Biol Chem. 1984; 259(4):2311-20. View

17.

Landry J, Sutton A, Tafrov S, Heller R, Stebbins J, Pillus L . The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases. Proc Natl Acad Sci U S A. 2000; 97(11):5807-11. PMC: 18515. DOI: 10.1073/pnas.110148297. View

18.

Kaeberlein M, Andalis A, Fink G, Guarente L . High osmolarity extends life span in Saccharomyces cerevisiae by a mechanism related to calorie restriction. Mol Cell Biol. 2002; 22(22):8056-66. PMC: 134739. DOI: 10.1128/MCB.22.22.8056-8066.2002. View

19.

Wei Y, Lin J, Frey P . 13C NMR analysis of electrostatic interactions between NAD+ and active site residues of UDP-galactose 4-epimerase: implications for the activation induced by uridine nucleotides. Biochemistry. 2001; 40(37):11279-87. DOI: 10.1021/bi011085z. View

20.

Sandmeier J, Celic I, Boeke J, Smith J . Telomeric and rDNA silencing in Saccharomyces cerevisiae are dependent on a nuclear NAD(+) salvage pathway. Genetics. 2002; 160(3):877-89. PMC: 1462005. DOI: 10.1093/genetics/160.3.877. View