Avidity Enhancement of L-selectin Bonds by Flow: Shear-promoted Rotation of Leukocytes Turn Labile Bonds into Functional Tethers

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2003 Nov 5

PMID 14597772

Citations 20

Authors

Oren Dwir

Ariel Solomon

Shmuel Mangan

Geoffrey S Kansas

Ulrich S Schwarz

Ronen Alon

Affiliations

Soon will be listed here.

Abstract

L-selectin is a key lectin essential for leukocyte capture and rolling on vessel walls. Functional adhesion of L-selectin requires a minimal threshold of hydrodynamic shear. Using high temporal resolution videomicroscopy, we now report that L-selectin engages its ligands through exceptionally labile adhesive bonds (tethers) even below this shear threshold. These tethers share a lifetime of 4 ms on distinct physiological ligands, two orders of magnitude shorter than the lifetime of the P-selectin-PSGL-1 bond. Below threshold shear, tether duration is not shortened by elevated shear stresses. However, above the shear threshold, selectin tethers undergo 14-fold stabilization by shear-driven leukocyte transport. Notably, the cytoplasmic tail of L-selectin contributes to this stabilization only above the shear threshold. These properties are not shared by P-selectin- or VLA-4-mediated tethers. L-selectin tethers appear adapted to undergo rapid avidity enhancement by cellular transport, a specialized mechanism not used by any other known adhesion receptor.

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