Production of Monoclonal Antibodies and Development of an Antigen Capture ELISA Directed Against the Envelope Glycoprotein GP of Ebola Virus

Overview

Journal Med Microbiol Immunol

Specialty Allergy & Immunology

Date 2003 Nov 1

PMID 14593476

Citations 18

Authors

Andreas Lucht

Roland Grunow

Christian Otterbein

Peggy Moller

Heinz Feldmann

Stephan Becker

Affiliations

Soon will be listed here.

Abstract

Ebola virus (EBOV) causes severe outbreaks of Ebola hemorrhagic fever in endemic regions of Africa and is considered to be of impact for other parts of the world as an imported viral disease. To develop a new diagnostic test, monoclonal antibodies to EBOV were produced from mice immunized with inactivated EBOV species Zaire. Antibodies directed against the viral glycoprotein GP were characterized by ELISA, Western blot and immunofluorescence analyses. An antigen capture ELISA was established, which is specific for EBOV-Zaire and shows a sensitivity of approximately 10(3) plaque-forming units/ml. Since the ELISA is able to detect even SDS-inactivated EBOV in spiked human sera, it could complement the existing diagnostic tools in the field and in routine laboratories where high containment facilities are not available.

Citing Articles

Self-amplifying RNA vaccine protects mice against lethal Ebola virus infection.

Krahling V, Erbar S, Kupke A, Nogueira S, Walzer K, Berger H Mol Ther. 2022; 31(2):374-386.

PMID: 36303436 PMC: 9931551. DOI: 10.1016/j.ymthe.2022.10.011.

Protective CD8+ T Cell Response Induced by Modified Vaccinia Virus Ankara Delivering Ebola Virus Nucleoprotein.

Kupke A, Volz A, Dietzel E, Freudenstein A, Schmidt J, Shams-Eldin H Vaccines (Basel). 2022; 10(4).

PMID: 35455282 PMC: 9027530. DOI: 10.3390/vaccines10040533.

Activating Natural Killer Cell Receptors, Selectins, and Inhibitory Siglecs Recognize Ebolavirus Glycoprotein.

Jarahian M, Marstaller K, Banna N, Ahani R, Etemadzadeh M, Boller L J Innate Immun. 2021; 14(2):135-147.

PMID: 34425576 PMC: 9082198. DOI: 10.1159/000517628.

Role of plasmonics in detection of deadliest viruses: a review.

Sohrabi F, Saeidifard S, Ghasemi M, Asadishad T, Hamidi S, Hosseini S Eur Phys J Plus. 2021; 136(6):675.

PMID: 34178567 PMC: 8214556. DOI: 10.1140/epjp/s13360-021-01657-9.

Simultaneous detection of Marburg virus and Ebola virus with TaqMan-based multiplex real-time PCR method.

Yu Z, Wu H, Huang Q, Zhong Z J Clin Lab Anal. 2021; 35(6):e23786.

PMID: 33939238 PMC: 8183904. DOI: 10.1002/jcla.23786.

References

Merzlikin N, Chepurnov A, Istomina N, Ofitserov V, Vorobeva M . [Development and application of an immunoenzyme test system for diagnosing Ebola fever]. Vopr Virusol. 1995; 40(1):31-5. View

Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495-7. DOI: 10.1038/256495a0. View

Sadek R, Khan A, Stevens G, Peters C, Ksiazek T . Ebola hemorrhagic fever, Democratic Republic of the Congo, 1995: determinants of survival. J Infect Dis. 1999; 179 Suppl 1:S24-7. DOI: 10.1086/514311. View

Volchkov V, Volchkova V, Slenczka W, Klenk H, Feldmann H . Release of viral glycoproteins during Ebola virus infection. Virology. 1998; 245(1):110-9. DOI: 10.1006/viro.1998.9143. View

Slenczka W, SHU H, Piepenburg G, Siegert R . ["Marburg virus" antigen demonstration by immunofluorescence in the organs of infected guinea pigs]. Dtsch Med Wochenschr. 1968; 93(12):612-6. DOI: 10.1055/s-0028-1105104. View

Rowe A, Bertolli J, Khan A, Mukunu R, Muyembe-Tamfum J, Bressler D . Clinical, virologic, and immunologic follow-up of convalescent Ebola hemorrhagic fever patients and their household contacts, Kikwit, Democratic Republic of the Congo. Commission de Lutte contre les Epidémies à Kikwit. J Infect Dis. 1999; 179 Suppl 1:S28-35. DOI: 10.1086/514318. View

Volchkov V, Feldmann H, Volchkova V, Klenk H . Processing of the Ebola virus glycoprotein by the proprotein convertase furin. Proc Natl Acad Sci U S A. 1998; 95(10):5762-7. PMC: 20453. DOI: 10.1073/pnas.95.10.5762. View

Kearney J, Radbruch A, Liesegang B, Rajewsky K . A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979; 123(4):1548-50. View

BOWEN E, Lloyd G, Harris W, Platt G, Baskerville A, Vella E . Viral haemorrhagic fever in southern Sudan and northern Zaire. Preliminary studies on the aetiological agent. Lancet. 1977; 1(8011):571-3. DOI: 10.1016/s0140-6736(77)92001-3. View

10.

van der Waals F, Pomeroy K, Goudsmit J, Asher D, Gajdusek D . Hemorrhagic fever virus infections in an isolated rainforest area of central Liberia. Limitations of the indirect immunofluorescence slide test for antibody screening in Africa. Trop Geogr Med. 1986; 38(3):209-14. View

11.

Borisevich I, Mikhailov V, Potryvaeva N, Malinkin I, Kirillov A, Krasnianskii V . [Development of the immunoenzyme test-system for detection of Ebola virus antigen]. Vopr Virusol. 1996; 41(5):232-4. View

12.

Leroy E, Baize S, Lu C, McCormick J, Georges A, Georges-Courbot M . Diagnosis of Ebola haemorrhagic fever by RT-PCR in an epidemic setting. J Med Virol. 2000; 60(4):463-7. View

13.

Niikura M, Ikegami T, Saijo M, Kurane I, Miranda M, Morikawa S . Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein. J Clin Microbiol. 2001; 39(9):3267-71. PMC: 88329. DOI: 10.1128/JCM.39.9.3267-3271.2001. View

14.

Jahrling P, Geisbert T, Dalgard D, Johnson E, Ksiazek T, Hall W . Preliminary report: isolation of Ebola virus from monkeys imported to USA. Lancet. 1990; 335(8688):502-5. DOI: 10.1016/0140-6736(90)90737-p. View

15.

Kiley M, BOWEN E, Eddy G, Isaacson M, Johnson K, McCormick J . Filoviridae: a taxonomic home for Marburg and Ebola viruses?. Intervirology. 1982; 18(1-2):24-32. DOI: 10.1159/000149300. View

16.

Rollin P, Ksiazek T, Jahrling P, HAINES M, Peters C . Detection of Ebola-like viruses by immunofluorescence. Lancet. 1990; 336(8730):1591. DOI: 10.1016/0140-6736(90)93378-3. View

17.

Maruyama T, Rodriguez L, Jahrling P, Sanchez A, Khan A, Nichol S . Ebola virus can be effectively neutralized by antibody produced in natural human infection. J Virol. 1999; 73(7):6024-30. PMC: 112663. DOI: 10.1128/JVI.73.7.6024-6030.1999. View

18.

Feldmann H, Volchkov V, Volchkova V, Stroher U, Klenk H . Biosynthesis and role of filoviral glycoproteins. J Gen Virol. 2001; 82(Pt 12):2839-2848. DOI: 10.1099/0022-1317-82-12-2839. View

19.

Felgner J, Kumar R, Sridhar C, Wheeler C, Tsai Y, Border R . Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations. J Biol Chem. 1994; 269(4):2550-61. View

20.

Okome-Nkoumou M, Kombila M . [A case of Ebola virus hemorrhagic fever in Libreville (Gabon), fatal after evacuation to South Africa]. Med Trop (Mars). 2000; 59(4):411. View