The Species-specific Egg Receptor for Sea Urchin Sperm Adhesion is EBR1,a Novel ADAMTS Protein

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2003 Oct 17

PMID 14561772

Citations 46

Authors

Noriko Kamei

Charles G Glabe

Affiliations

Soon will be listed here.

Abstract

Species-specific adhesion of sperm to the egg during sea urchin fertilization involves the interaction of the sperm adhesive protein,bindin, and a complementary receptor on the egg surface,and serves to restrict the gene pool to individuals of the same species. We used PCR representation difference analysis to clone the species-specific egg receptor for bindin, EBR1, from Strongylocentrotus franciscanus (Sf) and S. purpuratus (Sp). Sf-EBR1 contains a novel ADAMTS-like N-terminal domain followed by approximately 19 tandem EBR repeats consisting of alternating CUB and thrombospondin type 1 (TSP-1) domains where the last 10 EBR repeats are species-specific and highly conserved. Recombinant protein corresponding to the species-specific EBR repeat displays species-specific sperm adhesion and bindin-binding activity. The Sp-EBR1 ortholog has the same ADAMTS (a disintegrin and metalloprotease with thrombospondin type-1 modules) core region followed by eight and one-half tandem egg bindin receptor (EBR) repeats that share 88% identity with the Sf-EBR1 repeats,but has an entirely different species-specific domain consisting of hyalin-like (HYR) repeats. Thus,the species-specific domains of egg bindin receptor 1 (EBR1) from both species function as the egg surface receptor to mediate species-specific sperm adhesion.

Citing Articles

Identification of Six Novel Proteins Containing a ZP Module from Nemertean Species.

Ikenaga J, Yoshida K, Yoshida M Biomolecules. 2025; 14(12.

PMID: 39766251 PMC: 11673550. DOI: 10.3390/biom14121545.

The Effect of Synthetic Polyamine BPA-C8 on the Fertilization Process of Intact and Denuded Sea Urchin Eggs.

Limatola N, Chun J, Schmitt J, Lehn J, Santella L Cells. 2024; 13(17.

PMID: 39273047 PMC: 11394060. DOI: 10.3390/cells13171477.

SPE-51, a sperm-secreted protein with an immunoglobulin-like domain, is required for fertilization in C. elegans.

Mei X, Maniates K, Looper A, Krauchunas A, Druzhinina M, Dharia S Curr Biol. 2023; 33(14):3048-3055.e6.

PMID: 37453427 PMC: 10528068. DOI: 10.1016/j.cub.2023.06.029.

Divergent molecular signatures in fish Bouncer proteins define cross-fertilization boundaries.

Gert K, Panser K, Surm J, Steinmetz B, Schleiffer A, Jovine L Nat Commun. 2023; 14(1):3506.

PMID: 37316475 PMC: 10267171. DOI: 10.1038/s41467-023-39317-4.

Eukaryotic fertilization and gamete fusion at a glance.

Lu Y, Ikawa M J Cell Sci. 2022; 135(22).

PMID: 36416181 PMC: 10658792. DOI: 10.1242/jcs.260296.

References

Hubank M, Schatz D . Identifying differences in mRNA expression by representational difference analysis of cDNA. Nucleic Acids Res. 1994; 22(25):5640-8. PMC: 310128. DOI: 10.1093/nar/22.25.5640. View

Kuno K, Matsushima K . ADAMTS-1 protein anchors at the extracellular matrix through the thrombospondin type I motifs and its spacing region. J Biol Chem. 1998; 273(22):13912-7. DOI: 10.1074/jbc.273.22.13912. View

Bork P, Beckmann G . The CUB domain. A widespread module in developmentally regulated proteins. J Mol Biol. 1993; 231(2):539-45. DOI: 10.1006/jmbi.1993.1305. View

Kamei N, Swanson W, Glabe C . A rapidly diverging EGF protein regulates species-specific signal transduction in early sea urchin development. Dev Biol. 2000; 225(2):267-76. DOI: 10.1006/dbio.2000.9837. View

Burland T . DNASTAR's Lasergene sequence analysis software. Methods Mol Biol. 1999; 132:71-91. DOI: 10.1385/1-59259-192-2:71. View

Minor J, Britten R, Davidson E . Species-specific inhibition of fertilization by a peptide derived from the sperm protein bindin. Mol Biol Cell. 1993; 4(4):375-87. PMC: 300939. DOI: 10.1091/mbc.4.4.375. View

Glabe C, Vacquier V . Species specific agglutination of eggs by bindin isolated from sea urchin sperm. Nature. 1977; 267(5614):836-8. DOI: 10.1038/267836a0. View

Adams J . Thrombospondin-1. Int J Biochem Cell Biol. 1997; 29(6):861-5. DOI: 10.1016/s1357-2725(96)00171-9. View

Tang B . ADAMTS: a novel family of extracellular matrix proteases. Int J Biochem Cell Biol. 2001; 33(1):33-44. DOI: 10.1016/s1357-2725(00)00061-3. View

10.

Tang B, Hong W . ADAMTS: a novel family of proteases with an ADAM protease domain and thrombospondin 1 repeats. FEBS Lett. 1999; 445(2-3):223-5. DOI: 10.1016/s0014-5793(99)00119-2. View

11.

Vacquier V, Tegner M, Epel D . Protease released from sea urchin eggs at fertilization alters the vitelline layer and aids in preventing polyspermy. Exp Cell Res. 1973; 80(1):111-9. DOI: 10.1016/0014-4827(73)90281-4. View

12.

Lopo A, Glabe C, Lennarz W, Vacquier V . Sperm-egg binding events during sea urchin fertilization. Ann N Y Acad Sci. 1982; 383:405-25. DOI: 10.1111/j.1749-6632.1982.tb23181.x. View

13.

He Z, Brakebusch C, Fassler R, Kreidberg J, Primakoff P, Myles D . None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusion. Dev Biol. 2003; 254(2):226-37. DOI: 10.1016/s0012-1606(02)00043-x. View

14.

Adams J, Tucker R . The thrombospondin type 1 repeat (TSR) superfamily: diverse proteins with related roles in neuronal development. Dev Dyn. 2000; 218(2):280-99. DOI: 10.1002/(SICI)1097-0177(200006)218:2<280::AID-DVDY4>3.0.CO;2-0. View

15.

Bornstein P . Thrombospondins as matricellular modulators of cell function. J Clin Invest. 2001; 107(8):929-34. PMC: 199563. DOI: 10.1172/JCI12749. View

16.

Glabe C, Lennarz W . Isolation of a high molecular weight glycoconjugate derived from the surface of S purpuratus eggs that is implicated in sperm adhesion. J Supramol Struct Cell Biochem. 1981; 15(4):387-94. DOI: 10.1002/jsscb.1981.380150408. View

17.

Bode W, Gomis-Ruth F, Stockler W . Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc-binding environments (HEXXHXXGXXH and Met-turn) and topologies and should be grouped into a common family, the 'metzincins'. FEBS Lett. 1993; 331(1-2):134-40. DOI: 10.1016/0014-5793(93)80312-i. View

18.

Letunic I, Goodstadt L, Dickens N, Doerks T, Schultz J, Mott R . Recent improvements to the SMART domain-based sequence annotation resource. Nucleic Acids Res. 2001; 30(1):242-4. PMC: 99073. DOI: 10.1093/nar/30.1.242. View

19.

Primakoff P, Myles D . Penetration, adhesion, and fusion in mammalian sperm-egg interaction. Science. 2002; 296(5576):2183-5. DOI: 10.1126/science.1072029. View

20.

Earles D, Lennarz W . Identification and partial characterization of sperm receptor associated with the newly formed fertilization envelope from sea urchin eggs. Dev Biol. 1986; 117(1):204-8. DOI: 10.1016/0012-1606(86)90362-3. View