Differential Effects of Bcl-2 on T and B Cells in Transgenic Mice

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1992 Dec 1

PMID 1454823

Citations 45

Authors

M Katsumata

R M Siegel

D C Louie

T Miyashita

Y Tsujimoto

P C Nowell

M I Greene

J C Reed

Affiliations

Soon will be listed here.

Abstract

We have produced bcl-2 transgenic mice by using a construct which mimics the t(14;18) translocation in human follicular lymphomas. Although lymphoid tissues from all transgenic mice contained high levels of human Bcl-2 protein, transgene expression was differentially regulated within the B- and T-cell compartments of lines derived from various founder mice. We have characterized the phenotypes of two lines of bcl-2 transgenic mice (line 2 and line 6) in which bcl-2 transgene expression was restricted primarily to the T- or B-cell lineages, respectively. Analysis of line 6 lymphocytes revealed a polyclonal expansion of B cells, and these B cells exhibited prolonged survival in vitro. In line 2 mice, numbers of T cells in the peripheral lymphoid tissues were more moderately elevated despite enhanced T-cell survival in vitro. Line 2 transgenic mice also showed significantly increased proportions of thymocytes with a mature phenotype. Taken together, these findings suggest different roles for bcl-2 in the in vivo regulation of B- and T-cell development and homeostasis.

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