High Genetic Diversity of the Immunodominant Region of the Feline Calicivirus Capsid Gene in Endemically Infected Cat Colonies

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2003 Sep 23

PMID 14501192

Citations 21

Authors

Alan D Radford

Susan Dawson

Ruth Ryvar

Karen Coyne

Deborah R Johnson

Michael B Cox

Els F J Acke

Diane D Addie

Rosalind M Gaskell

Affiliations

Soon will be listed here.

Abstract

Feline calicivirus (FCV) is an important pathogen of domestic cats. In this study, we have determined the genetic diversity of FCV within four geographically separate colonies of endemically infected cats by sequencing the immunodominant and variable region E of the capsid gene. Comparison of isolates between colonies and between unrelated published sequences gave nucleotide distance values of 26-35% and 22-40%, respectively and suggested each colony was infected with a distinct virus strain. Comparison of isolates within individual endemically infected colonies showed nucleotide distance variability of 0-16%. This was greater than distances previously reported for epidemiologically related isolates from cases of acute disease (0-5%) and was consistent with the evolution of FCV from a single distinct ancestor sequence in each colony. The pattern of nucleotide substitutions generating the observed intra-colony diversity was associated with strong evidence for positive selection acting on immunodominant regions of the FCV capsid protein. We suggest that endemically infected colonies of cats may be important generators of genetic diversity for FCV and that this may ultimately lead to the generation of new strains.

Citing Articles

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