Cloning, Sequencing, and Expression of the Escherichia Coli Peptide Methionine Sulfoxide Reductase Gene

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 1992 Aug 5

PMID 1386361

Citations 31

Authors

M A Rahman

H Nelson

H Weissbach

N Brot

Affiliations

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Abstract

The gene encoding peptide methionine sulfoxide reductase was cloned from an Escherichia coli genomic library using an oligonucleotide probe based on the amino-terminal sequence of the protein. The nucleotide sequence revealed that the gene codes for a polypeptide of 212 amino acid residues with a calculated molecular weight of 23,314. The protein has been overexpressed in E. coli and is present as a soluble active species.

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