Interleukin 4 Inhibits in Vitro Proliferation of Leukemic and Normal Human B Cell Precursors

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1992 Nov 1

PMID 1385474

Citations 3

Authors

D Pandrau

S Saeland

V Duvert

I Durand

A M Manel

M T Zabot

N Philippe

J Banchereau

Affiliations

Soon will be listed here.

Abstract

In the present study, we have investigated the effects of IL-4 on the proliferation and differentiation of leukemic and normal human B cell precursors (BCP). We have demonstrated that IL-4 significantly inhibited spontaneous [3H]thymidine ([3H]-TdR) incorporation by leukemic blasts from some B lineage acute lymphoblastic leukemia (BCP-ALL) patients (8 of 14). Furthermore, IL-4 was found to suppress the spontaneous and factor-dependent (IL-7 and IL-3) proliferation of normal BCP (CD10+ surface [s] IgM- cells) isolated from fetal bone marrow. Maximum growth inhibition of either leukemic or normal BCP was reached at low IL-4 concentrations (10 U/ml), and the effect was specifically neutralized by anti-IL-4 antibody. IL-4 was further found to induce the expression of CD20 antigen on BCP-ALL cells from a number of the cases examined (5 of 8), but in contrast to leukemic cells, IL-4 failed to induce CD20 antigen on normal BCP. Finally, IL-4 was found to induce neither the expression of cytoplasmic mu chain, nor the appearance of sIgM+ cells in cultures of normal or leukemic BCP. Our data indicate that IL-4 has the potential to inhibit cell proliferation in leukemic and normal human B lymphopoiesis but is unable to drive the transition from BCP to mature B cells.

Citing Articles

Induction of interferon-stimulated genes on the IL-4 response axis by Epstein-Barr virus infected human b cells; relevance to cellular transformation.

Smith N, Tierney R, Wei W, Vockerodt M, Murray P, Woodman C PLoS One. 2013; 8(5):e64868.

PMID: 23724103 PMC: 3664578. DOI: 10.1371/journal.pone.0064868.

IL-7 sensitizes human pre-B cells but not pro-B cells to Fas/APO-1 (CD95)-mediated apoptosis.

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Human B cell precursors proliferate and express CD23 after CD40 ligation.

Saeland S, Duvert V, Moreau I, Banchereau J J Exp Med. 1993; 178(1):113-20.

PMID: 7686210 PMC: 2191089. DOI: 10.1084/jem.178.1.113.

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