Monocyte Attachment to Activated Human Vascular Endothelium in Vitro is Mediated by Leukocyte Adhesion Molecule-1 (L-selectin) Under Nonstatic Conditions

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1992 Jun 11

PMID 1375271

Citations 43

Authors

O Spertini

F W Luscinskas

M A Gimbrone Jr

T F Tedder

Affiliations

Soon will be listed here.

Abstract

The receptors that mediate monocyte adhesion to cytokine-stimulated endothelial monolayers were assessed using a nonstatic (rotating) cell-attachment assay. In this system, leukocyte adhesion molecule-1 (LAM-1) (L-selectin) mediated a major portion (87 +/- 15% at 37 degrees C) of monocyte attachment to activated endothelium. mAb blocking of endothelial leukocyte adhesion molecule-1 (41% inhibition), CD18 (36%), and vascular cell adhesion molecule-1 (25%) function had lesser effects on attachment. These results suggest that LAM-1 may serve an important role in monocyte attachment to endothelium at sites of inflammation.

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