Damage by Visible Light to the Acridine Orange--DNA Complex

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1961 May 1

PMID 13701685

Citations 22

Authors

D FREIFELDER

P F Davison

E P Geiduschek

Affiliations

Soon will be listed here.

Abstract

Salmon DNA has been irradiated with visible light in the presence of acridine orange. If the dye is bound to the DNA, there results: (a) a decrease in sedimentation coefficient, (b) a lowering of viscosity, and (c) a decrease in the thermal denaturation temperature. CsCl banding experiments show that the first two effects reflect depolymerization of the DNA. Depolymerization apparently occurs by single-strand scission although some double-strand scission is not excluded. The destabilization of secondary structure results probably from chemical attack on the components of the individual strands.

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References

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