A CD4+ Cytotoxic T-lymphocyte Clone to a Conserved Epitope on Human Immunodeficiency Virus Type 1 P24: Cytotoxic Activity and Secretion of Interleukin-2 and Interleukin-6

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1992 Jan 1

PMID 1370094

Citations 20

Authors

R A Littaua

M B Oldstone

A Takeda

F A Ennis

Affiliations

Soon will be listed here.

Abstract

A CD4+ cytotoxic T-lymphocyte (CTL) clone, established from the peripheral blood of a human immunodeficiency virus (HIV)-seropositive donor, lysed autologous target cells that were infected with a recombinant vaccinia virus containing the gag gene of HIV type 1 and target cells pulsed with p24gag construct expressed in Escherichia coli. The recognition of the HLA-DQ-restricted epitope by this clone was further defined by using overlapping synthetic peptides. The epitope recognized by this CD4+ CTL clone (amino acids 140 to 148) overlaps with a CD8+ epitope and is highly conserved among all isolates of HIV type 1 that have been sequenced. Production and secretion of lymphokines such as interleukin-2 and interleukin-6 after specific antigenic stimulation were demonstrated by this gag-specific CD4+ CTL clone.

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