Electron Microscopy of Salivary Gland Viruses

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1958 May 1

PMID 13525574

Citations 25

Authors

S A LUSE

M G Smith

Affiliations

Soon will be listed here.

Abstract

A human and a mouse strain of the salivary gland virus have been examined by electron microscopy. The human strain was transmitted, prior to examination, to tissue cultures derived from human myometrial cells, while the mouse strain was examined in mice inoculated intraperitoneally. The nuclear forms associated with both strains of virus were morphologically similar. Nuclear inclusions, composed of particles interspersed with dense clumped chromatin, were a striking feature of infected cells. The cytoplasmic forms were of 2 types-one a 300 to 500 mmicro homogeneous dense spherical form, and the other a target-like form composed of a central dense dot in a pale zone surrounded by a dense shell-the entire configuration measuring 100 to 180 mmicro. The target-like particle appeared to be identical in both strains. The spherical cytoplasmic forms in cells infected with the human strain appeared to be solid, while in cells infected with the mouse strain there was evidence of formation of target-like forms within the spheres. Possible mechanisms by which infection of the cell may occur, as well as possible mechanisms and sites of multiplication of virus, are discussed.

Citing Articles

Subviral Dense Bodies of Human Cytomegalovirus Induce an Antiviral Type I Interferon Response.

Penner I, Buscher N, Dejung M, Freiwald A, Butter F, Plachter B Cells. 2022; 11(24).

PMID: 36552792 PMC: 9777239. DOI: 10.3390/cells11244028.

Murine Cytomegalovirus Exploits Olfaction To Enter New Hosts.

Farrell H, Lawler C, Tan C, MacDonald K, Bruce K, Mach M mBio. 2016; 7(2):e00251-16.

PMID: 27118588 PMC: 4850257. DOI: 10.1128/mBio.00251-16.

Human cytomegalovirus tegument protein pUL71 is required for efficient virion egress.

Womack A, Shenk T mBio. 2010; 1(5).

PMID: 21151777 PMC: 2999941. DOI: 10.1128/mBio.00282-10.

Electron microscopic observations on the development of herpes simplex virus.

Morgan C, Rose H, Holden M, Jones E J Exp Med. 1959; 110:643-56.

PMID: 14424096 PMC: 2136996. DOI: 10.1084/jem.110.4.643.

MORPHOLOGICAL AND BIOLOGICAL STUDIES ON A VIRUS IN CULTURED LYMPHOBLASTS FROM BURKITT'S LYMPHOMA.

Epstein M, Henle G, ACHONG B, BARR Y J Exp Med. 1965; 121:761-70.

PMID: 14278230 PMC: 2138004. DOI: 10.1084/jem.121.5.761.

References

LATTA H, HARTMANN J . Use of a glass edge in thin sectioning for electron microscopy. Proc Soc Exp Biol Med. 1950; 74(2):436-9. DOI: 10.3181/00379727-74-17931. View

PORTER K, Blum J . A study in microtomy for electron microscopy. Anat Rec. 1953; 117(4):685-710. DOI: 10.1002/ar.1091170403. View

Smith M, VELLIOS F . Inclusion disease or generalized salivary gland virus infection. AMA Arch Pathol. 1950; 50(6):862-84. View

Fawcett D . Electron microscope observations on intracellular virus-like particles associated with the cells of the Lucké renal adenocarcinoma. J Biophys Biochem Cytol. 1956; 2(6):725-41. PMC: 2224004. DOI: 10.1083/jcb.2.6.725. View

ACKERMANN W, Kurtz H . The relation of herpes virus to host cell mitochondria. J Exp Med. 1952; 96(2):151-7. PMC: 2136132. DOI: 10.1084/jem.96.2.151. View

Cowdry E, Scott G . Nuclear Inclusions Suggestive of Virus Action in the Salivary Glands of the Monkey, Cebus Fatuellus. Am J Pathol. 2009; 11(4):647-658.1. PMC: 1910980. DOI: 10.3181/00379727-32-7828p. View

GAYLORD Jr W . Intracellular forms of meningopneumonitis virus. J Exp Med. 1954; 100(6):575-80. PMC: 2136399. DOI: 10.1084/jem.100.6.575. View

WYATT J, Saxton J . Generalized cytomegalic inclusion disease. J Pediatr. 1950; 36(3):271-94, illust. DOI: 10.1016/s0022-3476(50)80097-5. View

Morgan C, ELLISON S, Rose H, Moore D . Structure and development of viruses as observed in the electron microscope. I. Herpes simplex virus. J Exp Med. 1954; 100(2):195-202. PMC: 2136369. DOI: 10.1084/jem.100.2.195. View

10.

Rector E, Rector L . Intranuclear Inclusions in the Salivary Glands of Moles. Am J Pathol. 2009; 10(5):629-636.1. PMC: 2062881. View

11.

DEMPSEY E, LANSING A . Improved knife-holders for thin-sectioning with rotary microtomes. Proc Soc Exp Biol Med. 1953; 82(2):253-6. DOI: 10.3181/00379727-82-20082. View

12.

Friedlaender M, Moore D, Love R, BROWN R, KOPROWSKI H . Studies with the electron microscope of virus-host relationships in Ehrlich ascites tumor cells. I. The identification and structure of anopheles A virus. J Exp Med. 1955; 102(4):361-70. PMC: 2136519. DOI: 10.1084/jem.102.4.361. View

13.

SCHWERDT C, Pardee A . The intracellular distribution of Lansing poliomyelitis virus in the central nervous system of infected cotton rats. J Exp Med. 1952; 96(2):121-36. PMC: 2136133. DOI: 10.1084/jem.96.2.121. View

14.

MERCER R, LUSE S, GUYTON D . Clinical diagnosis of generalized cytomegalic inclusion disease. Pediatrics. 1953; 11(5):502-14. View

15.

KUTTNER A, Wang S . THE PROBLEM OF THE SIGNIFICANCE OF THE INCLUSION BODIES FOUND IN THE SALIVARY GLANDS OF INFANTS, AND THE OCCURRENCE OF INCLUSION BODIES IN THE SUBMAXILLARY GLANDS OF HAMSTERS, WHITE MICE, AND WILD RATS (PEIPING). J Exp Med. 2009; 60(6):773-91. PMC: 2132411. DOI: 10.1084/jem.60.6.773. View

16.

HAYMAKER W, GIRDANY B, Stephens J, LILLIE R, FETTERMAN G . Cerebral involvement with advanced periventricular calcification in generalized cytomegalic inclusion disease in the newborn; a clinicopathological report of a case diagnosed during life. J Neuropathol Exp Neurol. 1954; 13(4):562-86. DOI: 10.1093/jnen/13.4.562. View

17.

Reissig M, Melnick J . The cellular changes produced in tissue cultures by herpes B virus correlated with the concurrent multiplication of the virus. J Exp Med. 1955; 101(3):341-52. PMC: 2136472. DOI: 10.1084/jem.101.3.341. View

18.

Bang F . Cellular changes in the chick chorio-allantoic membrane infected with herpes simplex and vaccinia; a study with thin sections for the electron microscope. Bull Johns Hopkins Hosp. 1950; 87(6):511-47. View