Sparfloxacin, Ethambutol, and Cortisol Receptor Inhibitor RU-40 555 Treatment for Disseminated Mycobacterium Avium Complex Infection of Normal C57BL/6 Mice

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1992 Nov 1

PMID 1336944

Citations 8

Authors

C Perronne

Y Cohen

C Truffot-Pernot

J Grosset

J L Vilde

J J Pocidalo

Affiliations

Soon will be listed here.

Abstract

Sparfloxacin (50 mg/kg of body weight given subcutaneously each day), alone or in combination with ethambutol (50 mg/kg given subcutaneously each day), was examined for its therapeutic efficacy against experimental infection induced with the Mycobacterium avium complex in normal C57BL/6 mice. In addition, the potential anti-infective role of RU-40 555 (100 mg/kg given intraperitoneally each day), a drug that inhibits the cortisol receptors, was examined in the same model. Treatments were started 24 h after intravenous bacterial challenge and were continued for 21 days. Compared with controls, sparfloxacin or ethambutol decreased the CFU counts in spleens and lungs (P < 0.001). The sparfloxacin plus ethambutol combination was more effective than sparfloxacin alone in spleens (P < 0.001) but not in lungs. The sparfloxacin plus ethambutol plus RU-40 555 combination was more effective than the sparfloxacin plus ethambutol combination in spleens and lungs (P < 0.001). Thus, in this model, RU-40 555 enhanced the antibacterial activities of the antibiotics tested. Results of the study showed that normal C57BL/6 mice infected with the M. avium complex can be used for the evaluation of antimicrobial agents.

Citing Articles

Corticosteroid-induced immunosuppression ultimately does not compromise the efficacy of antibiotherapy in murine Mycobacterium ulcerans infection.

Martins T, Trigo G, Fraga A, Gama J, Longatto-Filho A, Saraiva M PLoS Negl Trop Dis. 2012; 6(11):e1925.

PMID: 23209864 PMC: 3510087. DOI: 10.1371/journal.pntd.0001925.

Drug evaluation of concurrent Pneumocystis carinii, Toxoplasma gondii, and Mycobacterium avium complex infections in a rat model.

Chau F, Bertrand G, Garry L, Derouin F, Girard P Antimicrob Agents Chemother. 1998; 42(5):1068-72.

PMID: 9593128 PMC: 105746. DOI: 10.1128/AAC.42.5.1068.

Mycobacterium avium complex infection in mice: lack of exacerbation after LP-BM5 murine leukemia virus infection.

Grassi F, Perronne C, Cohen Y, Maslo C, Chau F, Sinet M Infect Immun. 1996; 64(4):1203-7.

PMID: 8606079 PMC: 173904. DOI: 10.1128/iai.64.4.1203-1207.1996.

Clarithromycin, minocycline, and rifabutin treatments before and after infection of C57BL/6 mice with Mycobacterium avium.

Lazard T, Perronne C, Grosset J, Vilde J, Pocidalo J Antimicrob Agents Chemother. 1993; 37(8):1690-2.

PMID: 8215285 PMC: 188043. DOI: 10.1128/AAC.37.8.1690.

Effectiveness of various antimicrobial agents against Mycobacterium avium complex in the beige mouse model.

Ji B, Lounis N, Truffot-Pernot C, Grosset J Antimicrob Agents Chemother. 1994; 38(11):2521-9.

PMID: 7872741 PMC: 188235. DOI: 10.1128/AAC.38.11.2521.

References

Sternberg E, Hill J, Chrousos G, Kamilaris T, Listwak S, Gold P . Inflammatory mediator-induced hypothalamic-pituitary-adrenal axis activation is defective in streptococcal cell wall arthritis-susceptible Lewis rats. Proc Natl Acad Sci U S A. 1989; 86(7):2374-8. PMC: 286915. DOI: 10.1073/pnas.86.7.2374. View

Hirschelmann R, Schade R, Klingner E, BEKEMEIER H . Dexamethasone antagonism by RU 38486 in inflammatory reactions of the rat. Part 2: Biochemical parameters: RNA content of inflammation cells and acute phase reactants of the blood. Pharmazie. 1988; 43(5):370-1. View

Bermudez L, Young L . Activities of amikacin, roxithromycin, and azithromycin alone or in combination with tumor necrosis factor against Mycobacterium avium complex. Antimicrob Agents Chemother. 1988; 32(8):1149-53. PMC: 172367. DOI: 10.1128/AAC.32.8.1149. View

GANGADHARAM P, Perumal V, Jairam B, Rao P, Nguyen A, Farhi D . Activity of rifabutin alone or in combination with clofazimine or ethambutol or both against acute and chronic experimental Mycobacterium intracellulare infections. Am Rev Respir Dis. 1987; 136(2):329-33. DOI: 10.1164/ajrccm/136.2.329. View

Hirschelmann R, Klingner E, Schmidt K, BEKEMEIER H . Dexamethasone antagonism by RU 38,486 in inflammatory reactions of the rat and mouse. Part 1: Degree of inflammation. Pharmazie. 1988; 43(3):219-20. View

Lazar G, Agarwal M . The influence of a novel glucocorticoid antagonist on endotoxin lethality in mice strains. Biochem Med Metab Biol. 1986; 36(1):70-4. DOI: 10.1016/0885-4505(86)90108-8. View

Emilie D, Galanaud P, Baulieu E, Dormont J . Inhibition of in vitro immunosuppressive effects of glucocorticosteroids by a competitive antagonist RU-486. Immunol Lett. 1984; 8(4):183-6. DOI: 10.1016/0165-2478(84)90075-0. View

C J, P J . Hormone antagonist with broad potential. Science. 1989; 245(4924):1322. DOI: 10.1126/science.245.4924.1322. View

Dautzenberg B, Truffot C, Legris S, Meyohas M, Berlie H, Mercat A . Activity of clarithromycin against Mycobacterium avium infection in patients with the acquired immune deficiency syndrome. A controlled clinical trial. Am Rev Respir Dis. 1991; 144(3 Pt 1):564-9. DOI: 10.1164/ajrccm/144.3_Pt_1.564. View

10.

Horsburgh Jr C . Mycobacterium avium complex infection in the acquired immunodeficiency syndrome. N Engl J Med. 1991; 324(19):1332-8. DOI: 10.1056/NEJM199105093241906. View

11.

Ellner J, Goldberger M, Parenti D . Mycobacterium avium infection and AIDS: a therapeutic dilemma in rapid evolution. J Infect Dis. 1991; 163(6):1326-35. DOI: 10.1093/infdis/163.6.1326. View

12.

Furney S, Roberts A, Orme I . Effect of rifabutin on disseminated Mycobacterium avium infections in thymectomized, CD4 T-cell-deficient mice. Antimicrob Agents Chemother. 1990; 34(9):1629-32. PMC: 171895. DOI: 10.1128/AAC.34.9.1629. View

13.

Segal S . Mifepristone (RU 486). N Engl J Med. 1990; 322(10):691-3. DOI: 10.1056/NEJM199003083221009. View

14.

Rastogi N, Goh K, David H . Enhancement of drug susceptibility of Mycobacterium avium by inhibitors of cell envelope synthesis. Antimicrob Agents Chemother. 1990; 34(5):759-64. PMC: 171687. DOI: 10.1128/AAC.34.5.759. View

15.

Fernandes P, Hardy D, McDaniel D, Hanson C, SWANSON R . In vitro and in vivo activities of clarithromycin against Mycobacterium avium. Antimicrob Agents Chemother. 1989; 33(9):1531-4. PMC: 172696. DOI: 10.1128/AAC.33.9.1531. View

16.

Bermudez L, Stevens P, Kolonoski P, Wu M, Young L . Treatment of experimental disseminated Mycobacterium avium complex infection in mice with recombinant IL-2 and tumor necrosis factor. J Immunol. 1989; 143(9):2996-3000. View

17.

Baulieu E . Contragestion and other clinical applications of RU 486, an antiprogesterone at the receptor. Science. 1989; 245(4924):1351-7. DOI: 10.1126/science.2781282. View

18.

Laue L, Kawai S, Brandon D, Brightwell D, Barnes K, Knazek R . Receptor-mediated effects of glucocorticoids on inflammation: enhancement of the inflammatory response with a glucocorticoid antagonist. J Steroid Biochem. 1988; 29(6):591-8. DOI: 10.1016/0022-4731(88)90156-2. View

19.

Dietrich J, Chasserot-Golaz S, Beck G, Bauer G . Antagonism of glucocorticoid induction of Epstein-Barr virus early antigens by different steroids in Daudi lymphoma cells. J Steroid Biochem. 1986; 24(1):417-21. DOI: 10.1016/0022-4731(86)90093-2. View

20.

Formstecher P, Lefebvre P, DAUTREVAUX M . RU 486 stabilizes the glucocorticoid receptor in a non-transformed high molecular weight form in intact thymus cells under physiological conditions. J Steroid Biochem. 1988; 31(4B):607-12. DOI: 10.1016/0022-4731(88)90012-x. View