New Hepatocellular Carcinoma Cell Line SUHC-1 Established from a Patient with Hepatitis C Virus RNA in Serum

Overview

Journal Jpn J Cancer Res

Specialty Oncology

Date 1992 Aug 1

PMID 1328123

Authors

K Yoshizawa

K Kiyosawa

S Usuda

K Yabu

Y Nakatsuji

S Yamada

K Furuta

E Tanaka

T Sodeyama

S Furuta

Affiliations

Soon will be listed here.

Abstract

A new human hepatocellular carcinoma cell (HCC) line, designated SUHC-1, was derived from a Japanese patient with hepatocellular carcinoma having antibody to hepatitis C virus (HCV) and HCV-RNA in his serum, and established in tissue culture. This cell line exhibited typical epithelial cell morphology in culture as observed by phase-contrast and electron microscopy. The SUHC-1 cells produced albumin and alpha 2-macroglobulin. Chromosomal analysis showed several rearrangements at short and long arms of chromosome 1, 17 and 20 (1p-, 1q-, i(1q), i(17q) and 20q+) with a modal number of 91. HCV-RNA was not detected in the supernatant of SUHC-1 cells by nested polymerase chain reaction assay or in the SUHC-1 cells by the in situ hybridization method. We concluded that complete HCV does not exist in the SUHC-1 cell line. The SUHC-1 cell line is the first line of HCC to have been derived from a patient with persistent HCV infection, and may provide a suitable model for studies of hepatocarcinogenesis related to HCV.

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