An Epstein-Barr Virus Transformation-associated Membrane Protein Interacts with Src Family Tyrosine Kinases

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1992 Aug 1

PMID 1321296

Citations 56

Authors

A L Burkhardt

J B Bolen

E Kieff

R Longnecker

Affiliations

Soon will be listed here.

Abstract

In latently infected growth-transformed human lymphocytes, Epstein-Barr virus (EBV) encodes two integral plasma membrane proteins: LMP1, which constitutively induces B-lymphocyte activation and intercellular adhesion, and LMP2A, which associates with LMP1 and is a tyrosine kinase substrate. We now demonstrate that LMP2A associates with src family protein tyrosine kinases, particularly lyn kinase, in nonionic detergent extracts of transfected B lymphoma cells or in extracts of EBV-transformed B lymphocytes. The LMP2A and tyrosine kinase association is stable in nonionic detergents and includes a 70-kDa cell protein which is also an in vitro or in vivo kinase substrate. This LMP2A association with B-lymphocyte src family tyrosine kinases is likely to be an important pathway in EBV's effects on cell growth.

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