Wnt-5/pipetail Functions in Vertebrate Axis Formation As a Negative Regulator of Wnt/beta-catenin Activity

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2003 Sep 4

PMID 12952939

Citations 108

Authors

Trudi A Westfall

Ryan Brimeyer

Jen Twedt

Jean Gladon

Andrea Olberding

Makoto Furutani-Seiki

Diane C Slusarski

Affiliations

Soon will be listed here.

Abstract

We provide genetic evidence defining a role for noncanonical Wnt function in vertebrate axis formation. In zebrafish, misexpression of Wnt-4, -5, and -11 stimulates calcium (Ca2+) release, defining the Wnt/Ca2+ class. We describe genetic interaction between two Wnt/Ca2+ members, Wnt-5 (pipetail) and Wnt-11 (silberblick), and a reduction of Ca2+ release in Wnt-5/pipetail. Embryos genetically depleted of both maternal and zygotic Wnt-5 product exhibit cell movement defects as well as hyperdorsalization and axis-duplication phenotypes. The dorsalized phenotypes result from increased beta-catenin accumulation and activation of downstream genes. The Wnt-5 loss-of-function defect is consistent with Ca2+ modulation having an antagonistic interaction with Wnt/beta-catenin signaling.

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